data_7263 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Complete 1H assignment of Meg-A12 ; _BMRB_accession_number 7263 _BMRB_flat_file_name bmr7263.str _Entry_type original _Submission_date 2006-08-14 _Accession_date 2006-08-14 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wolf Christian A. . 2 Dancea Felician . . 3 Shi Meichen . . 4 Bade-Noskova Veronika . . 5 Rueterjans Heinz . . 6 Kerjaschki Dontscho . . 7 Luecke Christian . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 252 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2007-03-09 original author . stop_ _Original_release_date 2007-03-09 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution structure of the twelfth cysteine-rich ligand-binding repeat in rat megalin' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 17245526 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Wolf Christian A. . 2 Dancea Felician . . 3 Shi Meichen . . 4 Bade-Noskova Veronika . . 5 Rueterjans Heinz . . 6 Kerjaschki Dontscho . . 7 Luecke Christian . . stop_ _Journal_abbreviation 'J. Biomol. NMR' _Journal_volume 37 _Journal_issue 4 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 321 _Page_last 328 _Year 2007 _Details . loop_ _Keyword 'calcium cage' 'cysteine-rich repeat' 'ligand-binding domain' 'low density lipoprotein receptor' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Meg-A12 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Meg-A12 $Meg-A12 'CALCIUM (II) ION' $CA stop_ _System_molecular_weight 5092.67 _System_physical_state native _System_oligomer_state protein _System_paramagnetic no _System_thiol_state 'all disulfide bound' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Meg-A12 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Meg-A12 _Molecular_mass 5092.67 _Mol_thiol_state 'all disulfide bound' loop_ _Biological_function 'ligand binding domain' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 48 _Mol_residue_sequence ; GAMVLNCTSAQFKCADGSSC INSRYRCDGVYDCRDNSDEA GCPTRPPG ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1182 GLY 2 1183 ALA 3 1184 MET 4 1185 VAL 5 1186 LEU 6 1187 ASN 7 1188 CYS 8 1189 THR 9 1190 SER 10 1191 ALA 11 1192 GLN 12 1193 PHE 13 1194 LYS 14 1195 CYS 15 1196 ALA 16 1197 ASP 17 1198 GLY 18 1199 SER 19 1200 SER 20 1201 CYS 21 1202 ILE 22 1203 ASN 23 1204 SER 24 1205 ARG 25 1206 TYR 26 1207 ARG 27 1208 CYS 28 1209 ASP 29 1210 GLY 30 1211 VAL 31 1212 TYR 32 1213 ASP 33 1214 CYS 34 1215 ARG 35 1216 ASP 36 1217 ASN 37 1218 SER 38 1219 ASP 39 1220 GLU 40 1221 ALA 41 1222 GLY 42 1223 CYS 43 1224 PRO 44 1225 THR 45 1226 ARG 46 1227 PRO 47 1228 PRO 48 1229 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2014-05-12 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2I1P "Solution Structure Of The Twelfth Cysteine-Rich Ligand- Binding Repeat In Rat Megalin" 100.00 48 100.00 100.00 7.50e-26 stop_ save_ ############# # Ligands # ############# save_CA _Saveframe_category ligand _Mol_type non-polymer _Name_common "CA (CALCIUM ION)" _BMRB_code . _PDB_code CA _Molecular_mass 40.078 _Mol_charge 2 _Mol_paramagnetic . _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Fri Sep 30 12:55:05 2011 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CA CA CA . 2 . ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Meg-A12 Rat 10116 Eukaryota Metazoa Rattus norvegicus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $Meg-A12 'recombinant technology' 'E. coli' . . . plasmid pKM263 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $assembly 1.5 mM . stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name xwinnmr _Version 2.6 loop_ _Vendor _Address _Electronic_address BRUKER . . stop_ loop_ _Task 'Data collection' stop_ _Details . save_ save_software_2 _Saveframe_category software _Name AURELIA _Version 2.5.9 loop_ _Vendor _Address _Electronic_address BRUKER . . stop_ loop_ _Task 'Data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer BRUKER _Model DMX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H1H_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1H1H TOCSY' _Sample_label $sample_1 save_ save_1H1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '1H1H NOESY' _Sample_label $sample_1 save_ save_1H1H_TOCSY _Saveframe_category NMR_applied_experiment _Experiment_name '1H1H TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_1H1H_NOESY _Saveframe_category NMR_applied_experiment _Experiment_name '1H1H NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.5 0.1 pH temperature 298 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '1H1H TOCSY' '1H1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name Meg-A12 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1182 1 GLY HA2 H 4.21 0.01 9 2 1182 1 GLY HA3 H 4.21 0.01 9 3 1183 2 ALA H H 8.50 0.01 1 4 1183 2 ALA HA H 4.32 0.01 1 5 1183 2 ALA HB H 1.50 0.01 1 6 1184 3 MET H H 8.42 0.01 1 7 1184 3 MET HA H 4.49 0.01 1 8 1184 3 MET HB2 H 2.02 0.01 1 9 1184 3 MET HB3 H 2.02 0.01 1 10 1184 3 MET HG2 H 2.56 0.01 1 11 1184 3 MET HG3 H 2.56 0.01 1 12 1185 4 VAL H H 8.31 0.01 1 13 1185 4 VAL HA H 4.09 0.01 1 14 1185 4 VAL HB H 1.98 0.01 1 15 1185 4 VAL HG1 H 0.88 0.01 1 16 1185 4 VAL HG2 H 0.88 0.01 1 17 1186 5 LEU H H 8.39 0.01 1 18 1186 5 LEU HA H 4.33 0.01 1 19 1186 5 LEU HB2 H 1.53 0.01 2 20 1186 5 LEU HB3 H 1.49 0.01 2 21 1186 5 LEU HG H 1.43 0.01 1 22 1186 5 LEU HD1 H 0.87 0.01 2 23 1186 5 LEU HD2 H 0.74 0.01 2 24 1187 6 ASN H H 8.44 0.01 1 25 1187 6 ASN HA H 4.89 0.01 1 26 1187 6 ASN HB2 H 2.73 0.01 2 27 1187 6 ASN HB3 H 2.62 0.01 2 28 1187 6 ASN HD21 H 7.61 0.01 2 29 1187 6 ASN HD22 H 6.92 0.01 2 30 1188 7 CYS H H 8.50 0.01 1 31 1188 7 CYS HA H 5.07 0.01 1 32 1188 7 CYS HB2 H 3.43 0.01 2 33 1188 7 CYS HB3 H 2.59 0.01 2 34 1189 8 THR H H 8.76 0.01 1 35 1189 8 THR HA H 4.57 0.01 1 36 1189 8 THR HB H 4.71 0.01 1 37 1189 8 THR HG2 H 1.25 0.01 1 38 1190 9 SER H H 8.83 0.01 1 39 1190 9 SER HA H 4.34 0.01 1 40 1190 9 SER HB2 H 4.09 0.01 2 41 1190 9 SER HB3 H 4.00 0.01 2 42 1191 10 ALA H H 7.78 0.01 1 43 1191 10 ALA HA H 4.60 0.01 1 44 1191 10 ALA HB H 1.40 0.01 1 45 1192 11 GLN H H 7.69 0.01 1 46 1192 11 GLN HA H 5.04 0.01 1 47 1192 11 GLN HB2 H 2.27 0.01 1 48 1192 11 GLN HB3 H 1.77 0.01 1 49 1192 11 GLN HG2 H 2.33 0.01 1 50 1192 11 GLN HG3 H 2.04 0.01 1 51 1192 11 GLN HE21 H 7.55 0.01 2 52 1192 11 GLN HE22 H 6.69 0.01 2 53 1193 12 PHE H H 9.41 0.01 1 54 1193 12 PHE HA H 4.72 0.01 1 55 1193 12 PHE HB2 H 3.00 0.01 2 56 1193 12 PHE HB3 H 2.63 0.01 2 57 1193 12 PHE HD1 H 7.12 0.01 1 58 1193 12 PHE HD2 H 7.12 0.01 1 59 1193 12 PHE HE1 H 7.43 0.01 1 60 1193 12 PHE HE2 H 7.43 0.01 1 61 1193 12 PHE HZ H 7.18 0.01 1 62 1194 13 LYS H H 7.89 0.01 1 63 1194 13 LYS HA H 4.49 0.01 1 64 1194 13 LYS HB2 H 1.52 0.01 1 65 1194 13 LYS HB3 H 1.52 0.01 1 66 1194 13 LYS HG2 H 1.27 0.01 2 67 1194 13 LYS HG3 H 1.16 0.01 2 68 1194 13 LYS HD2 H 1.40 0.01 1 69 1194 13 LYS HD3 H 1.40 0.01 1 70 1194 13 LYS HE2 H 2.84 0.01 1 71 1194 13 LYS HE3 H 2.84 0.01 1 72 1195 14 CYS H H 8.02 0.01 1 73 1195 14 CYS HA H 4.59 0.01 1 74 1195 14 CYS HB2 H 3.36 0.01 2 75 1195 14 CYS HB3 H 2.64 0.01 2 76 1196 15 ALA H H 9.38 0.01 1 77 1196 15 ALA HA H 3.94 0.01 1 78 1196 15 ALA HB H 1.60 0.01 1 79 1197 16 ASP H H 8.31 0.01 1 80 1197 16 ASP HA H 4.36 0.01 1 81 1197 16 ASP HB2 H 3.02 0.01 2 82 1197 16 ASP HB3 H 2.77 0.01 2 83 1198 17 GLY H H 8.85 0.01 1 84 1198 17 GLY HA2 H 4.10 0.01 2 85 1198 17 GLY HA3 H 3.44 0.01 2 86 1199 18 SER H H 8.15 0.01 1 87 1199 18 SER HA H 4.01 0.01 1 88 1199 18 SER HB2 H 3.81 0.01 1 89 1199 18 SER HB3 H 3.81 0.01 1 90 1200 19 SER H H 6.79 0.01 1 91 1200 19 SER HA H 4.49 0.01 1 92 1200 19 SER HB2 H 3.81 0.01 1 93 1200 19 SER HB3 H 3.81 0.01 1 94 1201 20 CYS H H 8.44 0.01 1 95 1201 20 CYS HA H 5.37 0.01 1 96 1201 20 CYS HB2 H 2.79 0.01 2 97 1201 20 CYS HB3 H 2.69 0.01 2 98 1202 21 ILE H H 9.28 0.01 1 99 1202 21 ILE HA H 5.02 0.01 1 100 1202 21 ILE HB H 2.12 0.01 1 101 1202 21 ILE HG12 H 1.64 0.01 2 102 1202 21 ILE HG13 H 1.45 0.01 2 103 1202 21 ILE HG2 H 0.91 0.01 1 104 1202 21 ILE HD1 H 0.95 0.01 1 105 1203 22 ASN H H 9.16 0.01 1 106 1203 22 ASN HA H 4.46 0.01 1 107 1203 22 ASN HB2 H 2.80 0.01 2 108 1203 22 ASN HB3 H 2.57 0.01 2 109 1203 22 ASN HD21 H 7.70 0.01 2 110 1203 22 ASN HD22 H 7.06 0.01 2 111 1204 23 SER H H 8.75 0.01 1 112 1204 23 SER HA H 3.89 0.01 1 113 1204 23 SER HB2 H 3.69 0.01 1 114 1204 23 SER HB3 H 3.69 0.01 1 115 1205 24 ARG H H 8.58 0.01 1 116 1205 24 ARG HA H 4.14 0.01 1 117 1205 24 ARG HB2 H 1.56 0.01 2 118 1205 24 ARG HB3 H 1.39 0.01 2 119 1205 24 ARG HG2 H 1.15 0.01 2 120 1205 24 ARG HG3 H 0.85 0.01 2 121 1205 24 ARG HD2 H 2.95 0.01 1 122 1205 24 ARG HD3 H 2.95 0.01 1 123 1205 24 ARG HE H 6.90 0.01 1 124 1206 25 TYR H H 7.87 0.01 1 125 1206 25 TYR HA H 4.89 0.01 1 126 1206 25 TYR HB2 H 3.89 0.01 2 127 1206 25 TYR HB3 H 2.75 0.01 2 128 1206 25 TYR HD1 H 7.27 0.01 1 129 1206 25 TYR HD2 H 7.27 0.01 1 130 1206 25 TYR HE1 H 6.85 0.01 1 131 1206 25 TYR HE2 H 6.85 0.01 1 132 1207 26 ARG H H 7.78 0.01 1 133 1207 26 ARG HA H 4.07 0.01 1 134 1207 26 ARG HB2 H 2.20 0.01 2 135 1207 26 ARG HB3 H 1.22 0.01 2 136 1207 26 ARG HG2 H 1.49 0.01 2 137 1207 26 ARG HG3 H 1.35 0.01 2 138 1207 26 ARG HD2 H 2.96 0.01 2 139 1207 26 ARG HD3 H 1.93 0.01 2 140 1207 26 ARG HE H 7.07 0.01 1 141 1208 27 CYS H H 8.48 0.01 1 142 1208 27 CYS HA H 4.89 0.01 9 143 1208 27 CYS HB2 H 3.34 0.01 2 144 1208 27 CYS HB3 H 3.13 0.01 2 145 1209 28 ASP H H 9.66 0.01 1 146 1209 28 ASP HA H 4.89 0.01 1 147 1209 28 ASP HB2 H 3.12 0.01 2 148 1209 28 ASP HB3 H 2.50 0.01 2 149 1210 29 GLY H H 9.32 0.01 1 150 1210 29 GLY HA2 H 4.07 0.01 2 151 1210 29 GLY HA3 H 3.35 0.01 2 152 1211 30 VAL H H 7.42 0.01 1 153 1211 30 VAL HA H 3.91 0.01 1 154 1211 30 VAL HB H 2.12 0.01 1 155 1211 30 VAL HG1 H 0.71 0.01 1 156 1211 30 VAL HG2 H 0.71 0.01 1 157 1212 31 TYR H H 8.98 0.01 1 158 1212 31 TYR HA H 4.73 0.01 1 159 1212 31 TYR HB2 H 3.22 0.01 2 160 1212 31 TYR HB3 H 2.99 0.01 2 161 1212 31 TYR HD1 H 6.96 0.01 1 162 1212 31 TYR HD2 H 6.96 0.01 1 163 1212 31 TYR HE1 H 6.82 0.01 1 164 1212 31 TYR HE2 H 6.82 0.01 1 165 1213 32 ASP H H 10.39 0.01 1 166 1213 32 ASP HA H 4.72 0.01 1 167 1213 32 ASP HB2 H 2.57 0.01 1 168 1213 32 ASP HB3 H 2.57 0.01 1 169 1214 33 CYS H H 8.50 0.01 1 170 1214 33 CYS HA H 4.85 0.01 9 171 1214 33 CYS HB2 H 3.62 0.01 2 172 1214 33 CYS HB3 H 3.33 0.01 2 173 1215 34 ARG H H 9.54 0.01 1 174 1215 34 ARG HA H 4.18 0.01 1 175 1215 34 ARG HB2 H 2.00 0.01 2 176 1215 34 ARG HB3 H 1.94 0.01 2 177 1215 34 ARG HG2 H 1.76 0.01 2 178 1215 34 ARG HG3 H 1.65 0.01 2 179 1215 34 ARG HD2 H 3.20 0.01 1 180 1215 34 ARG HD3 H 3.20 0.01 1 181 1215 34 ARG HE H 7.57 0.01 1 182 1216 35 ASP H H 7.69 0.01 1 183 1216 35 ASP HA H 4.50 0.01 1 184 1216 35 ASP HB2 H 3.09 0.01 2 185 1216 35 ASP HB3 H 2.58 0.01 2 186 1217 36 ASN H H 8.19 0.01 1 187 1217 36 ASN HA H 4.30 0.01 1 188 1217 36 ASN HB2 H 3.13 0.01 2 189 1217 36 ASN HB3 H 2.82 0.01 2 190 1217 36 ASN HD21 H 7.55 0.01 2 191 1217 36 ASN HD22 H 6.82 0.01 2 192 1218 37 SER H H 8.72 0.01 1 193 1218 37 SER HA H 4.13 0.01 1 194 1218 37 SER HB2 H 4.05 0.01 1 195 1218 37 SER HB3 H 4.05 0.01 1 196 1219 38 ASP H H 10.03 0.01 1 197 1219 38 ASP HA H 3.96 0.01 1 198 1219 38 ASP HB2 H 2.89 0.01 2 199 1219 38 ASP HB3 H 2.50 0.01 2 200 1220 39 GLU H H 7.58 0.01 1 201 1220 39 GLU HA H 4.86 0.01 1 202 1220 39 GLU HB2 H 2.11 0.01 2 203 1220 39 GLU HB3 H 1.60 0.01 2 204 1220 39 GLU HG2 H 2.59 0.01 1 205 1220 39 GLU HG3 H 2.59 0.01 1 206 1221 40 ALA H H 7.21 0.01 1 207 1221 40 ALA HA H 4.64 0.01 1 208 1221 40 ALA HB H 1.52 0.01 1 209 1222 41 GLY H H 9.05 0.01 1 210 1222 41 GLY HA2 H 3.98 0.01 2 211 1222 41 GLY HA3 H 3.87 0.01 2 212 1223 42 CYS H H 8.03 0.01 1 213 1223 42 CYS HA H 4.72 0.01 1 214 1223 42 CYS HB2 H 3.18 0.01 2 215 1223 42 CYS HB3 H 2.77 0.01 2 216 1224 43 PRO HA H 4.55 0.01 1 217 1224 43 PRO HB2 H 2.36 0.01 2 218 1224 43 PRO HB3 H 1.96 0.01 2 219 1224 43 PRO HG2 H 2.09 0.01 1 220 1224 43 PRO HG3 H 2.09 0.01 1 221 1224 43 PRO HD2 H 3.95 0.01 2 222 1224 43 PRO HD3 H 3.78 0.01 2 223 1225 44 THR H H 8.34 0.01 1 224 1225 44 THR HA H 4.24 0.01 1 225 1225 44 THR HB H 4.13 0.01 1 226 1225 44 THR HG2 H 1.22 0.01 1 227 1226 45 ARG H H 8.42 0.01 1 228 1226 45 ARG HA H 4.66 0.01 1 229 1226 45 ARG HB2 H 1.83 0.01 2 230 1226 45 ARG HB3 H 1.72 0.01 2 231 1226 45 ARG HG2 H 1.68 0.01 1 232 1226 45 ARG HG3 H 1.68 0.01 1 233 1226 45 ARG HD2 H 3.19 0.01 1 234 1226 45 ARG HD3 H 3.19 0.01 1 235 1226 45 ARG HE H 7.21 0.01 1 236 1227 46 PRO HA H 4.72 0.01 1 237 1227 46 PRO HB2 H 2.39 0.01 2 238 1227 46 PRO HB3 H 1.94 0.01 2 239 1227 46 PRO HG2 H 2.05 0.01 1 240 1227 46 PRO HG3 H 2.05 0.01 1 241 1227 46 PRO HD2 H 3.86 0.01 2 242 1227 46 PRO HD3 H 3.61 0.01 2 243 1228 47 PRO HA H 4.44 0.01 1 244 1228 47 PRO HB2 H 2.32 0.01 2 245 1228 47 PRO HB3 H 2.02 0.01 2 246 1228 47 PRO HG2 H 2.07 0.01 1 247 1228 47 PRO HG3 H 2.07 0.01 1 248 1228 47 PRO HD2 H 3.84 0.01 2 249 1228 47 PRO HD3 H 3.71 0.01 2 250 1229 48 GLY H H 7.97 0.01 1 251 1229 48 GLY HA2 H 3.77 0.01 1 252 1229 48 GLY HA3 H 3.77 0.01 1 stop_ save_