data_96 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy ; _BMRB_accession_number 96 _BMRB_flat_file_name bmr96.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-04-13 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zuiderweg Erik R.P. . 2 Kaptein Robert . . 3 Wuthrich Kurt . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 194 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-10 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-04-13 revision BMRB 'Link to the Protein Data Bank added' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Zuiderweg, Erik R. P., Kaptein, Robert, Wuthrich, Kurt, "Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy," Eur. J. Biochem. 137, 279-292 (1983). ; _Citation_title ; Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Zuiderweg Erik R.P. . 2 Kaptein Robert . . 3 Wuthrich Kurt . . stop_ _Journal_abbreviation 'Eur. J. Biochem.' _Journal_volume 137 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 279 _Page_last 292 _Year 1983 _Details . save_ ################################## # Molecular system description # ################################## save_system_lac_repressor_headpiece _Saveframe_category molecular_system _Mol_system_name 'lac repressor headpiece' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'lac repressor headpiece' $lac_repressor_headpiece stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_lac_repressor_headpiece _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'lac repressor headpiece' _Name_variant HP51 _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 51 _Mol_residue_sequence ; MKPVTLYDVAEYAGVSYQTV SRVVNQASHVSAKTREKVEA AMAELNYIPNR ; loop_ _Residue_seq_code _Residue_label 1 MET 2 LYS 3 PRO 4 VAL 5 THR 6 LEU 7 TYR 8 ASP 9 VAL 10 ALA 11 GLU 12 TYR 13 ALA 14 GLY 15 VAL 16 SER 17 TYR 18 GLN 19 THR 20 VAL 21 SER 22 ARG 23 VAL 24 VAL 25 ASN 26 GLN 27 ALA 28 SER 29 HIS 30 VAL 31 SER 32 ALA 33 LYS 34 THR 35 ARG 36 GLU 37 LYS 38 VAL 39 GLU 40 ALA 41 ALA 42 MET 43 ALA 44 GLU 45 LEU 46 ASN 47 TYR 48 ILE 49 PRO 50 ASN 51 ARG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1066 "lac repressor headpiece" 100.00 60 100.00 100.00 1.61e-27 BMRB 127 "lac repressor headpiece" 100.00 51 100.00 100.00 2.26e-27 BMRB 1494 "lac repressor headpiece" 100.00 56 100.00 100.00 1.84e-27 BMRB 1552 "lac repressor headpiece" 100.00 51 100.00 100.00 2.26e-27 BMRB 2956 "lac repressor headpiece" 100.00 56 98.04 98.04 1.99e-26 BMRB 32 "lac repressor headpiece" 100.00 51 100.00 100.00 2.26e-27 BMRB 4813 "LAC REPRESSOR" 100.00 62 100.00 100.00 1.38e-27 BMRB 5345 "lactose repressor-operator" 100.00 62 100.00 100.00 1.27e-27 BMRB 5791 "Lactose operon repressor" 100.00 62 100.00 100.00 1.27e-27 BMRB 661 "lac repressor headpiece" 100.00 60 100.00 100.00 1.61e-27 BMRB 736 "lac repressor headpiece" 100.00 56 100.00 100.00 1.84e-27 BMRB 848 "lac repressor headpiece" 100.00 51 100.00 100.00 2.26e-27 BMRB 849 "lac repressor headpiece" 100.00 51 100.00 100.00 2.26e-27 PDB 1CJG "Nmr Structure Of Lac Repressor Hp62-Dna Complex" 100.00 62 100.00 100.00 1.38e-27 PDB 1EFA "Crystal Structure Of The Lac Repressor Dimer Bound To Operator And The Anti-Inducer Onpf" 100.00 333 100.00 100.00 8.95e-26 PDB 1JWL "Structure Of The Dimeric Lac RepressorOPERATOR O1ONPF Complex" 100.00 333 100.00 100.00 8.95e-26 PDB 1JYE "Structure Of A Dimeric Lac Repressor With C-Terminal Deletion And K84l Substitution" 100.00 349 100.00 100.00 8.57e-26 PDB 1JYF "Structure Of The Dimeric Lac Repressor With An 11-Residue C-Terminal Deletion" 100.00 349 100.00 100.00 8.91e-26 PDB 1L1M "Solution Structure Of A Dimer Of Lac Repressor Dna-Binding Domain Complexed To Its Natural Operator O1" 100.00 62 100.00 100.00 1.27e-27 PDB 1LBG "Lactose Operon Repressor Bound To 21-Base Pair Symmetric Operator Dna, Alpha Carbons Only" 100.00 360 100.00 100.00 8.74e-26 PDB 1LBH "Intact Lactose Operon Repressor With Gratuitous Inducer Iptg" 100.00 360 100.00 100.00 8.74e-26 PDB 1LBI "Lac Repressor" 100.00 360 100.00 100.00 8.74e-26 PDB 1LCC "Structure Of The Complex Of Lac Repressor Headpiece And An 11 Base-pair Half-operator Determined By Nuclear Magnetic Resonance " 100.00 51 100.00 100.00 2.26e-27 PDB 1LCD "Structure Of The Complex Of Lac Repressor Headpiece And An 11 Base-pair Half-operator Determined By Nuclear Magnetic Resonance " 100.00 51 100.00 100.00 2.26e-27 PDB 1LQC "Lac Repressor Headpiece (Residues 1-56), Nmr, 32 Structures" 100.00 56 100.00 100.00 1.84e-27 PDB 1OSL "Solution Structure Of A Dimeric Lactose Dna-Binding Domain Complexed To A Nonspecific Dna Sequence" 100.00 62 100.00 100.00 1.27e-27 PDB 2KEI "Refined Solution Structure Of A Dimer Of Lac Repressor Dna- Binding Domain Complexed To Its Natural Operator O1" 100.00 62 100.00 100.00 1.27e-27 PDB 2KEJ "Solution Structure Of A Dimer Of Lac Repressor Dna-Binding Domain Complexed To Its Natural Operator O2" 100.00 62 100.00 100.00 1.27e-27 PDB 2KEK "Solution Structure Of A Dimer Of Lac Repressor Dna-Binding Domain Complexed To Its Natural Operator O3" 100.00 62 100.00 100.00 1.27e-27 PDB 2PE5 "Crystal Structure Of The Lac Repressor Bound To Onpg In Repressed State" 98.04 330 100.00 100.00 5.78e-25 PDB 3EDC "Crystal Structure Of A 1.6-Hexanediol Bound Tetrameric Form Of Escherichia Coli Lac-Repressor Refined To 2.1 Resolution" 100.00 360 100.00 100.00 8.40e-26 DBJ BAB20667 "LacI [Cloning vector pCA24N]" 100.00 360 100.00 100.00 8.40e-26 DBJ BAB33821 "transcriptional repressor of the lac operon [Escherichia coli O157:H7 str. Sakai]" 100.00 360 100.00 100.00 8.49e-26 DBJ BAD00175 "LacI repressor protein [Cloning vector pGETS109]" 100.00 360 100.00 100.00 8.74e-26 DBJ BAD20286 "lactose repressor [Expression vector pCop-H1]" 100.00 360 100.00 100.00 8.40e-26 DBJ BAD20288 "lactose repressor [Expression vector pCop-H2]" 100.00 360 100.00 100.00 8.40e-26 EMBL CAA07594 "lac repressor [Cloning vector pEH1]" 100.00 360 100.00 100.00 8.66e-26 EMBL CAA07597 "lac repressor [Cloning vector pEH3]" 100.00 360 100.00 100.00 8.66e-26 EMBL CAA11118 "lacI [synthetic construct]" 100.00 360 100.00 100.00 8.40e-26 EMBL CAA11119 "lacI [synthetic construct]" 100.00 360 100.00 100.00 8.40e-26 EMBL CAA11120 "lacI [synthetic construct]" 100.00 360 100.00 100.00 8.40e-26 GB AAA24052 "lac repressor protein (gtg start codon) [Escherichia coli]" 100.00 360 100.00 100.00 8.66e-26 GB AAA56744 "lac repressor protein [unidentified cloning vector]" 100.00 360 100.00 100.00 8.40e-26 GB AAA56768 "lac operon repressor [Cloning vector pSIT]" 100.00 360 100.00 100.00 8.66e-26 GB AAA57088 "lac repressor [unidentified cloning vector]" 100.00 360 100.00 100.00 8.40e-26 GB AAA57091 "lac repressor [unidentified cloning vector]" 100.00 360 100.00 100.00 8.40e-26 REF NP_286086 "lac repressor [Escherichia coli O157:H7 str. EDL933]" 100.00 360 100.00 100.00 8.57e-26 REF NP_308425 "lac repressor [Escherichia coli O157:H7 str. Sakai]" 100.00 363 98.04 100.00 5.34e-25 REF NP_414879 "lactose-inducible lac operon transcriptional repressor [Escherichia coli str. K-12 substr. MG1655]" 100.00 360 100.00 100.00 8.40e-26 REF WP_000248559 "lac repressor [Escherichia sp. TW11588]" 100.00 363 98.04 100.00 6.31e-25 REF WP_000248563 "lac repressor [Escherichia coli]" 100.00 363 98.04 100.00 5.66e-25 SP P03023 "RecName: Full=Lactose operon repressor [Escherichia coli K-12]" 100.00 360 100.00 100.00 8.40e-26 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $lac_repressor_headpiece 'Shingella dysenteriae' 300267 Bacteria . Shingella dysenteriae Sd197 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 4.95 . na temperature 291 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H . . ppm 0 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'lac repressor headpiece' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 3 PRO HA H 4.53 . 1 2 . 3 PRO HB2 H 1.88 . 2 3 . 3 PRO HB3 H 2.31 . 2 4 . 4 VAL H H 8.37 . 1 5 . 4 VAL HA H 4.37 . 1 6 . 4 VAL HB H 2.14 . 1 7 . 4 VAL HG1 H 1.12 . 2 8 . 4 VAL HG2 H 1.16 . 2 9 . 5 THR H H 9.52 . 1 10 . 5 THR HA H 5.15 . 1 11 . 5 THR HB H 4.97 . 1 12 . 5 THR HG2 H 1.42 . 1 13 . 6 LEU H H 9.12 . 1 14 . 6 LEU HA H 3.68 . 1 15 . 6 LEU HB2 H 1.2 . 2 16 . 6 LEU HB3 H 1.8 . 2 17 . 6 LEU HG H 1.73 . 1 18 . 6 LEU HD1 H .3 . 2 19 . 6 LEU HD2 H .77 . 2 20 . 7 TYR HA H 4.18 . 1 21 . 7 TYR HB2 H 2.85 . 2 22 . 7 TYR HB3 H 3.12 . 2 23 . 7 TYR HD1 H 7.06 . 1 24 . 7 TYR HD2 H 7.06 . 1 25 . 7 TYR HE1 H 6.49 . 1 26 . 7 TYR HE2 H 6.49 . 1 27 . 8 ASP H H 7.57 . 1 28 . 8 ASP HA H 4.48 . 1 29 . 8 ASP HB2 H 2.75 . 2 30 . 8 ASP HB3 H 3.2 . 2 31 . 9 VAL H H 7.54 . 1 32 . 9 VAL HA H 3.3 . 1 33 . 9 VAL HB H 2.1 . 1 34 . 9 VAL HG1 H .59 . 2 35 . 9 VAL HG2 H .72 . 2 36 . 10 ALA H H 8.1 . 1 37 . 10 ALA HA H 3.67 . 1 38 . 10 ALA HB H 1.55 . 1 39 . 11 GLU H H 8 . 1 40 . 11 GLU HA H 4.1 . 1 41 . 12 TYR H H 8.17 . 1 42 . 12 TYR HA H 4.13 . 1 43 . 12 TYR HB2 H 3 . 2 44 . 12 TYR HB3 H 3.24 . 2 45 . 12 TYR HD1 H 7.08 . 1 46 . 12 TYR HD2 H 7.08 . 1 47 . 12 TYR HE1 H 6.82 . 1 48 . 12 TYR HE2 H 6.82 . 1 49 . 13 ALA H H 8.57 . 1 50 . 13 ALA HA H 4.3 . 1 51 . 13 ALA HB H 1.34 . 1 52 . 14 GLY H H 8.02 . 1 53 . 14 GLY HA2 H 3.94 . 1 54 . 14 GLY HA3 H 3.94 . 1 55 . 15 VAL H H 7.83 . 1 56 . 15 VAL HA H 4.8 . 1 57 . 15 VAL HB H 2.22 . 1 58 . 15 VAL HG1 H .69 . 2 59 . 15 VAL HG2 H .84 . 2 60 . 16 SER H H 8.58 . 1 61 . 16 SER HA H 4.5 . 1 62 . 16 SER HB2 H 4.08 . 2 63 . 16 SER HB3 H 4.46 . 2 64 . 17 TYR H H 9.23 . 1 65 . 17 TYR HA H 3.78 . 1 66 . 17 TYR HB2 H 2.89 . 2 67 . 17 TYR HB3 H 3.04 . 2 68 . 17 TYR HD1 H 6.49 . 1 69 . 17 TYR HD2 H 6.49 . 1 70 . 17 TYR HE1 H 6.43 . 1 71 . 17 TYR HE2 H 6.43 . 1 72 . 18 GLN H H 8.69 . 1 73 . 18 GLN HA H 3.8 . 1 74 . 19 THR H H 8 . 1 75 . 19 THR HA H 3.79 . 1 76 . 19 THR HB H 4.35 . 1 77 . 19 THR HG2 H 1.05 . 1 78 . 20 VAL H H 7.69 . 1 79 . 20 VAL HA H 3.32 . 1 80 . 20 VAL HB H 1.93 . 1 81 . 20 VAL HG1 H .71 . 2 82 . 20 VAL HG2 H .8 . 2 83 . 21 SER H H 8.24 . 1 84 . 21 SER HA H 3.75 . 1 85 . 21 SER HB2 H 3.51 . 2 86 . 21 SER HB3 H 3.61 . 2 87 . 22 ARG H H 7.88 . 1 88 . 22 ARG HA H 4.03 . 1 89 . 23 VAL H H 7.83 . 1 90 . 23 VAL HA H 3.57 . 1 91 . 23 VAL HB H 2.33 . 1 92 . 23 VAL HG1 H .93 . 2 93 . 23 VAL HG2 H .99 . 2 94 . 24 VAL H H 8.17 . 1 95 . 24 VAL HA H 3.75 . 1 96 . 24 VAL HB H 2.1 . 1 97 . 24 VAL HG1 H .95 . 2 98 . 24 VAL HG2 H .97 . 2 99 . 25 ASN H H 8.11 . 1 100 . 25 ASN HA H 4.74 . 1 101 . 25 ASN HB2 H 2.77 . 2 102 . 25 ASN HB3 H 2.88 . 2 103 . 26 GLN H H 8.18 . 1 104 . 26 GLN HA H 4.25 . 1 105 . 27 ALA H H 8.34 . 1 106 . 27 ALA HA H 4.25 . 1 107 . 27 ALA HB H 1.44 . 1 108 . 28 SER H H 8.23 . 1 109 . 28 SER HA H 4.33 . 1 110 . 28 SER HB2 H 3.7 . 2 111 . 28 SER HB3 H 3.8 . 2 112 . 29 HIS H H 8.68 . 1 113 . 29 HIS HA H 4.6 . 1 114 . 29 HIS HB2 H 3.24 . 2 115 . 29 HIS HB3 H 3.4 . 2 116 . 29 HIS HD2 H 7.28 . 1 117 . 29 HIS HE1 H 8.6 . 1 118 . 30 VAL H H 7.73 . 1 119 . 30 VAL HA H 4.29 . 1 120 . 30 VAL HB H 1.96 . 1 121 . 30 VAL HG1 H .82 . 2 122 . 30 VAL HG2 H .96 . 2 123 . 31 SER H H 8.88 . 1 124 . 31 SER HA H 4.37 . 1 125 . 31 SER HB2 H 4.04 . 2 126 . 31 SER HB3 H 4.26 . 2 127 . 32 ALA H H 8.96 . 1 128 . 32 ALA HA H 4.04 . 1 129 . 32 ALA HB H 1.49 . 1 130 . 33 LYS H H 8.43 . 1 131 . 33 LYS HA H 4.08 . 1 132 . 34 THR H H 7.66 . 1 133 . 34 THR HA H 3.84 . 1 134 . 34 THR HB H 4.18 . 1 135 . 34 THR HG2 H 1.05 . 1 136 . 38 VAL H H 8.13 . 1 137 . 38 VAL HA H 3.44 . 1 138 . 38 VAL HB H 2.22 . 1 139 . 38 VAL HG1 H .87 . 2 140 . 38 VAL HG2 H .92 . 2 141 . 39 GLU H H 9 . 1 142 . 39 GLU HA H 4.12 . 1 143 . 40 ALA H H 8.4 . 1 144 . 40 ALA HA H 4.3 . 1 145 . 40 ALA HB H 1.56 . 1 146 . 41 ALA H H 7.6 . 1 147 . 41 ALA HA H 4.11 . 1 148 . 41 ALA HB H 1.71 . 1 149 . 42 MET H H 8.37 . 1 150 . 42 MET HA H 3.72 . 1 151 . 43 ALA H H 7.78 . 1 152 . 43 ALA HA H 4.26 . 1 153 . 43 ALA HB H 1.54 . 1 154 . 44 GLU H H 8.32 . 1 155 . 44 GLU HA H 4.01 . 1 156 . 45 LEU H H 7.65 . 1 157 . 45 LEU HA H 4.18 . 1 158 . 45 LEU HB2 H 1.33 . 1 159 . 45 LEU HB3 H 1.33 . 1 160 . 45 LEU HG H 1.27 . 1 161 . 45 LEU HD1 H .46 . 2 162 . 45 LEU HD2 H .51 . 2 163 . 46 ASN H H 7.76 . 1 164 . 46 ASN HA H 4.28 . 1 165 . 46 ASN HB2 H 2.72 . 2 166 . 46 ASN HB3 H 3.13 . 2 167 . 46 ASN HD21 H 6.8 . 2 168 . 46 ASN HD22 H 7.43 . 2 169 . 47 TYR H H 8.42 . 1 170 . 47 TYR HA H 4.08 . 1 171 . 47 TYR HB2 H 2.68 . 2 172 . 47 TYR HB3 H 2.59 . 2 173 . 47 TYR HD1 H 6.78 . 1 174 . 47 TYR HD2 H 6.78 . 1 175 . 47 TYR HE1 H 6.64 . 1 176 . 47 TYR HE2 H 6.64 . 1 177 . 48 ILE HB H 1.58 . 1 178 . 48 ILE HG12 H 1 . 2 179 . 48 ILE HG13 H 1.4 . 2 180 . 48 ILE HG2 H .78 . 1 181 . 48 ILE HD1 H .74 . 1 182 . 49 PRO HA H 4.26 . 1 183 . 49 PRO HB2 H 1.93 . 2 184 . 49 PRO HB3 H 2.3 . 2 185 . 50 ASN H H 8.46 . 1 186 . 50 ASN HA H 4.64 . 1 187 . 50 ASN HB2 H 2.86 . 2 188 . 50 ASN HB3 H 2.76 . 2 189 . 50 ASN HD21 H 6.96 . 2 190 . 50 ASN HD22 H 7.68 . 2 191 . 51 ARG H H 7.9 . 1 192 . 51 ARG HA H 4.2 . 1 193 . 51 ARG HB2 H 1.7 . 1 194 . 51 ARG HB3 H 1.7 . 1 stop_ save_