data_4377 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 4377 _Entry.Title ; The Solution Structure of the Type X Cellulose Binding Domain from Pseudomonas xylanase A ; _Entry.Type . _Entry.Version_type original _Entry.Submission_date 1999-08-17 _Entry.Accession_date 1999-08-17 _Entry.Last_release_date 2000-03-02 _Entry.Original_release_date 2000-03-02 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 2.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Srinivasarao Raghothama . . . 4377 2 Peter Simpson . J . 4377 3 L Szabo . . . 4377 4 T Nagy . . . 4377 5 Harry Gilbert . J . 4377 6 Mike Williamson . P . 4377 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 4377 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '1H chemical shifts' 308 4377 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2000-03-02 1999-08-17 original author . 4377 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 4377 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code 20120586 _Citation.DOI . _Citation.PubMed_ID 10653641 _Citation.Full_citation . _Citation.Title ; Solution structure of the CBM10 cellulose binding module from Pseudomonas Xylanase A ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Biochemistry _Citation.Journal_name_full Biochemistry _Citation.Journal_volume 39 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 978 _Citation.Page_last 984 _Citation.Year 2000 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Srinivasarao Raghothama . . . 4377 1 2 Peter Simpson . J . 4377 1 3 L Szabo . . . 4377 1 4 T Nagy . . . 4377 1 5 Harry Gilbert . J . 4377 1 6 Mike Williamson . P . 4377 1 stop_ save_ save_ref._1 _Citation.Sf_category citations _Citation.Sf_framecode ref._1 _Citation.Entry_ID 4377 _Citation.ID 2 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 7717975 _Citation.Full_citation ; Ferreira, LMA, Durrant, AJ, Hall, J, Hazlewood, GP, Gilbert, HJ (1990) Biochem. J., 307, 191-195 ; _Citation.Title 'A modular xylanase containing a novel non-catalytic xylan-specific binding domain.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Biochem. J.' _Citation.Journal_name_full 'The Biochemical journal' _Citation.Journal_volume '307 ( Pt 1)' _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN 0264-6021 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 191 _Citation.Page_last 195 _Citation.Year 1995 _Citation.Details ; Xylanase D (XYLD) from Cellulomonas fimi contains a C-terminal cellulose-binding domain (CBD) and an internal domain that exhibits 65% sequence identity with the C-terminal CBD. Full-length XYLD binds to both cellulose and xylan. Deletion of the C-terminal CBD from XYLD abolishes the capacity of the enzyme to bind to cellulose, although the truncated xylanase retains its xylan-binding properties. A derivative of XYLD lacking both the C-terminal CBD and the internal CBD homologue did not bind to either cellulose or xylan. A fusion protein consisting of the XYLD internal CBD homologue linked to the C-terminus of glutathione S-transferase (GST) bound to xylan, but not to cellulose, while GST bound to neither of the polysaccharides. The Km and specific activity of full-length XYLD and truncated derivatives of the enzyme lacking the C-terminal CBD (XYLDcbd), and both the CBD and the internal CBD homologue (XYLDcd), were determined with soluble and insoluble xylan as the substrates. The data showed that the specific activities of the three enzymes were similar for both substrates, as were the Km values for soluble substrate. However, the Km values of XYLD and XYLDcbd for insoluble xylan were significantly lower than the Km of XYLDcd. Overall, these data indicate that the internal CBD homologue in XYLD constitutes a discrete xylan-binding domain which influences the affinity of the enzyme for insoluble xylan but does not directly affect the catalytic activity of the xylanase. The rationale for the evolution of this domain is discussed. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 'G W' Black G. W. . 4377 2 2 'G P' Hazlewood G. P. . 4377 2 3 'S J' Millward-Sadler S. J. . 4377 2 4 'J I' Laurie J. I. . 4377 2 5 'H J' Gilbert H. J. . 4377 2 stop_ save_ save_ref._2 _Citation.Sf_category citations _Citation.Sf_framecode ref._2 _Citation.Entry_ID 4377 _Citation.ID 3 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 2507868 _Citation.Full_citation ; Hall, J, Hazlewood, GP, Huskisson, NS, Durrant, AJ, Gilbert, HJ (1989) Mol. Microbiol., 3, 1211-1219 ; _Citation.Title 'Conserved serine-rich sequences in xylanase and cellulase from Pseudomonas fluorescens subspecies cellulosa: internal signal sequence and unusual protein processing.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Mol. Microbiol.' _Citation.Journal_name_full 'Molecular microbiology' _Citation.Journal_volume 3 _Citation.Journal_issue 9 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0950-382X _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1211 _Citation.Page_last 1219 _Citation.Year 1989 _Citation.Details ; The complete nucleotide sequence of the xynA gene coding for a xylanase (XYLA) expressed by Pseudomonas fluorescens subspecies cellulosa, has been determined. The structural gene consists of an open reading frame of 1833 bp followed by a TAA stop codon. Confirmation of the nucleotide sequence was obtained by comparing the predicted amino acid sequence with that derived by N-terminal analysis of purified forms of the xylanase. The signal peptide present at the N terminus of mature XYLA closely resembles signal peptides of other secreted proteins. Truncated forms of the xylanase gene, in which the sequence encoding the N-terminal signal peptide had been deleted, still expressed coli. XYLA contains domains which are homologous to an endoglucanase expressed by the same organism. These structures include serine-rich sequences. Bal31 deletions of xynA revealed the extent to which these conserved sequences, in XYLA, were essential for xylanase activity. Downstream of the TAA stop codon is a G + C-rich region of dyad symmetry (delta G = 24 kcal) characteristic of E. coli Rho-independent transcription terminators. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 J Hall J. . . 4377 3 2 'G P' Hazlewood G. P. . 4377 3 3 'N S' Huskisson N. S. . 4377 3 4 'A J' Durrant A. J. . 4377 3 5 'H J' Gilbert H. J. . 4377 3 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_system_CBDX _Assembly.Sf_category assembly _Assembly.Sf_framecode system_CBDX _Assembly.Entry_ID 4377 _Assembly.ID 1 _Assembly.Name 'type X cellulose binding domain from Pseudomonas xylanase A' _Assembly.BMRB_code . _Assembly.Number_of_components . _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state 'all disulfide bound' _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Assembly_type.Type _Assembly_type.Entry_ID _Assembly_type.Assembly_ID monomer 4377 1 stop_ loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 CBDX 1 $CBDX . . . native . . . . . 4377 1 stop_ loop_ _Bond.ID _Bond.Type _Bond.Value_order _Bond.Assembly_atom_ID_1 _Bond.Entity_assembly_ID_1 _Bond.Entity_assembly_name_1 _Bond.Entity_ID_1 _Bond.Comp_ID_1 _Bond.Comp_index_ID_1 _Bond.Seq_ID_1 _Bond.Atom_ID_1 _Bond.Assembly_atom_ID_2 _Bond.Entity_assembly_ID_2 _Bond.Entity_assembly_name_2 _Bond.Entity_ID_2 _Bond.Comp_ID_2 _Bond.Comp_index_ID_2 _Bond.Seq_ID_2 _Bond.Atom_ID_2 _Bond.Auth_entity_assembly_ID_1 _Bond.Auth_entity_assembly_name_1 _Bond.Auth_seq_ID_1 _Bond.Auth_comp_ID_1 _Bond.Auth_atom_ID_1 _Bond.Auth_entity_assembly_ID_2 _Bond.Auth_entity_assembly_name_2 _Bond.Auth_seq_ID_2 _Bond.Auth_comp_ID_2 _Bond.Auth_atom_ID_2 _Bond.Entry_ID _Bond.Assembly_ID 1 disulfide single . 1 . 1 CYS 35 35 SG . 1 . 1 CYS 50 50 SG . . . . . . . . . . 4377 1 2 disulfide single . 1 . 1 CYS 25 25 SG . 1 . 1 CYS 56 56 SG . . . . . . . . . . 4377 1 stop_ loop_ _Entity_deleted_atom.ID _Entity_deleted_atom.Entity_atom_list_ID _Entity_deleted_atom.Entity_assembly_ID _Entity_deleted_atom.Entity_ID _Entity_deleted_atom.Comp_ID _Entity_deleted_atom.Comp_index_ID _Entity_deleted_atom.Seq_ID _Entity_deleted_atom.Atom_ID _Entity_deleted_atom.Auth_entity_assembly_ID _Entity_deleted_atom.Auth_seq_ID _Entity_deleted_atom.Auth_comp_ID _Entity_deleted_atom.Auth_atom_ID _Entity_deleted_atom.Entry_ID _Entity_deleted_atom.Assembly_ID 1 . 1 1 CYS 35 35 HG . . . . 4377 1 2 . 1 1 CYS 50 50 HG . . . . 4377 1 3 . 1 1 CYS 25 25 HG . . . . 4377 1 4 . 1 1 CYS 56 56 HG . . . . 4377 1 stop_ loop_ _Assembly_db_link.Author_supplied _Assembly_db_link.Database_code _Assembly_db_link.Accession_code _Assembly_db_link.Entry_mol_code _Assembly_db_link.Entry_mol_name _Assembly_db_link.Entry_experimental_method _Assembly_db_link.Entry_structure_resolution _Assembly_db_link.Entry_relation_type _Assembly_db_link.Entry_details _Assembly_db_link.Entry_ID _Assembly_db_link.Assembly_ID yes PDB 1E8R . 'Chain A, Solution Structure Of Type X Cbd' . . . . 4377 1 yes PDB 1QLD . 'Chain A, Solution Structure Of Type X Cbm' . . . . 4377 1 stop_ loop_ _Assembly_common_name.Name _Assembly_common_name.Type _Assembly_common_name.Entry_ID _Assembly_common_name.Assembly_ID CBDX abbreviation 4377 1 'type X cellulose binding domain from Pseudomonas xylanase A' system 4377 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_CBDX _Entity.Sf_category entity _Entity.Sf_framecode CBDX _Entity.Entry_ID 4377 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name 'type X cellulose binding domain from Pseudomonas xylanase A' _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; HHHHHHHHHHSSGHIAGRHM GNQQCNWYGTLYPLCVTTTN GWGWEDQRSCIARSTCAAQP APFGIVGSGHHHHHH ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states . _Entity.Ambiguous_chem_comp_sites . _Entity.Nstd_monomer . _Entity.Nstd_chirality . _Entity.Nstd_linkage . _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 75 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic . _Entity.Thiol_state . _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-24 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no PDB 1E8R . "Solution Structure Of Type X Cbd" . . . . . 66.67 50 100.00 100.00 1.26e-29 . . . . 4377 1 2 no PDB 1QLD . "Solution Structure Of Type X Cbm" . . . . . 66.67 50 100.00 100.00 1.26e-29 . . . . 4377 1 stop_ loop_ _Entity_common_name.Name _Entity_common_name.Type _Entity_common_name.Entry_ID _Entity_common_name.Entity_ID CBDX abbreviation 4377 1 'type X cellulose binding domain from Pseudomonas xylanase A' common 4377 1 Xyl10A variant 4377 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . HIS . 4377 1 2 . HIS . 4377 1 3 . HIS . 4377 1 4 . HIS . 4377 1 5 . HIS . 4377 1 6 . HIS . 4377 1 7 . HIS . 4377 1 8 . HIS . 4377 1 9 . HIS . 4377 1 10 . HIS . 4377 1 11 . SER . 4377 1 12 . SER . 4377 1 13 . GLY . 4377 1 14 . HIS . 4377 1 15 . ILE . 4377 1 16 . ALA . 4377 1 17 . GLY . 4377 1 18 . ARG . 4377 1 19 . HIS . 4377 1 20 . MET . 4377 1 21 . GLY . 4377 1 22 . ASN . 4377 1 23 . GLN . 4377 1 24 . GLN . 4377 1 25 . CYS . 4377 1 26 . ASN . 4377 1 27 . TRP . 4377 1 28 . TYR . 4377 1 29 . GLY . 4377 1 30 . THR . 4377 1 31 . LEU . 4377 1 32 . TYR . 4377 1 33 . PRO . 4377 1 34 . LEU . 4377 1 35 . CYS . 4377 1 36 . VAL . 4377 1 37 . THR . 4377 1 38 . THR . 4377 1 39 . THR . 4377 1 40 . ASN . 4377 1 41 . GLY . 4377 1 42 . TRP . 4377 1 43 . GLY . 4377 1 44 . TRP . 4377 1 45 . GLU . 4377 1 46 . ASP . 4377 1 47 . GLN . 4377 1 48 . ARG . 4377 1 49 . SER . 4377 1 50 . CYS . 4377 1 51 . ILE . 4377 1 52 . ALA . 4377 1 53 . ARG . 4377 1 54 . SER . 4377 1 55 . THR . 4377 1 56 . CYS . 4377 1 57 . ALA . 4377 1 58 . ALA . 4377 1 59 . GLN . 4377 1 60 . PRO . 4377 1 61 . ALA . 4377 1 62 . PRO . 4377 1 63 . PHE . 4377 1 64 . GLY . 4377 1 65 . ILE . 4377 1 66 . VAL . 4377 1 67 . GLY . 4377 1 68 . SER . 4377 1 69 . GLY . 4377 1 70 . HIS . 4377 1 71 . HIS . 4377 1 72 . HIS . 4377 1 73 . HIS . 4377 1 74 . HIS . 4377 1 75 . HIS . 4377 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . HIS 1 1 4377 1 . HIS 2 2 4377 1 . HIS 3 3 4377 1 . HIS 4 4 4377 1 . HIS 5 5 4377 1 . HIS 6 6 4377 1 . HIS 7 7 4377 1 . HIS 8 8 4377 1 . HIS 9 9 4377 1 . HIS 10 10 4377 1 . SER 11 11 4377 1 . SER 12 12 4377 1 . GLY 13 13 4377 1 . HIS 14 14 4377 1 . ILE 15 15 4377 1 . ALA 16 16 4377 1 . GLY 17 17 4377 1 . ARG 18 18 4377 1 . HIS 19 19 4377 1 . MET 20 20 4377 1 . GLY 21 21 4377 1 . ASN 22 22 4377 1 . GLN 23 23 4377 1 . GLN 24 24 4377 1 . CYS 25 25 4377 1 . ASN 26 26 4377 1 . TRP 27 27 4377 1 . TYR 28 28 4377 1 . GLY 29 29 4377 1 . THR 30 30 4377 1 . LEU 31 31 4377 1 . TYR 32 32 4377 1 . PRO 33 33 4377 1 . LEU 34 34 4377 1 . CYS 35 35 4377 1 . VAL 36 36 4377 1 . THR 37 37 4377 1 . THR 38 38 4377 1 . THR 39 39 4377 1 . ASN 40 40 4377 1 . GLY 41 41 4377 1 . TRP 42 42 4377 1 . GLY 43 43 4377 1 . TRP 44 44 4377 1 . GLU 45 45 4377 1 . ASP 46 46 4377 1 . GLN 47 47 4377 1 . ARG 48 48 4377 1 . SER 49 49 4377 1 . CYS 50 50 4377 1 . ILE 51 51 4377 1 . ALA 52 52 4377 1 . ARG 53 53 4377 1 . SER 54 54 4377 1 . THR 55 55 4377 1 . CYS 56 56 4377 1 . ALA 57 57 4377 1 . ALA 58 58 4377 1 . GLN 59 59 4377 1 . PRO 60 60 4377 1 . ALA 61 61 4377 1 . PRO 62 62 4377 1 . PHE 63 63 4377 1 . GLY 64 64 4377 1 . ILE 65 65 4377 1 . VAL 66 66 4377 1 . GLY 67 67 4377 1 . SER 68 68 4377 1 . GLY 69 69 4377 1 . HIS 70 70 4377 1 . HIS 71 71 4377 1 . HIS 72 72 4377 1 . HIS 73 73 4377 1 . HIS 74 74 4377 1 . HIS 75 75 4377 1 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 4377 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $CBDX . 29436 . . 'Pseudomonas fluorescens' 'P. fluorescens' . . Eubacteria . Pseudomonas fluorescens cellulosa . . . . . . . . . . . . . . . . . . . . 4377 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 4377 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $CBDX . 'recombinant technology' 'Escherichia coli' 'E. coli' . . Escherichia coli BL21 . . . . . . . . . . . . . . . . . . . . . . 4377 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Sample.Sf_category sample _Sample.Sf_framecode sample_one _Sample.Entry_ID 4377 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system . _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'type X cellulose binding domain from Pseudomonas xylanase A' . . . 1 $CBDX . . 1 . . mM . . . . 4377 1 stop_ save_ ####################### # Sample conditions # ####################### save_experimental_conditions_one _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode experimental_conditions_one _Sample_condition_list.Entry_ID 4377 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH* 4.5 0.2 n/a 4377 1 temperature 323 1 K 4377 1 stop_ save_ ############################ # Computer software used # ############################ save_FELIX _Software.Sf_category software _Software.Sf_framecode FELIX _Software.Entry_ID 4377 _Software.ID 1 _Software.Name FELIX _Software.Version 97.0 _Software.Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_one _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_one _NMR_spectrometer.Entry_ID 4377 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_NMR_spectrometer_two _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer_two _NMR_spectrometer.Entry_ID 4377 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode spectrometer_list _NMR_spectrometer_list.Entry_ID 4377 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 NMR_spectrometer_one Bruker DRX . 500 . . . 4377 1 2 NMR_spectrometer_two Bruker DRX . 600 . . . 4377 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 4377 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 Homonuclear . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4377 1 2 TOCSY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4377 1 3 NOESY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4377 1 4 DQFC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4377 1 5 E.COSY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4377 1 stop_ save_ save_NMR_spec_expt__0_1 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_1 _NMR_spec_expt.Entry_ID 4377 _NMR_spec_expt.ID 1 _NMR_spec_expt.Name Homonuclear _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID . _NMR_spec_expt.NMR_spectrometer_label . _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID . _NMR_spec_expt.Software_label . _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details . save_ save_NMR_spec_expt__0_2 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_2 _NMR_spec_expt.Entry_ID 4377 _NMR_spec_expt.ID 2 _NMR_spec_expt.Name TOCSY _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID . _NMR_spec_expt.NMR_spectrometer_label . _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID . _NMR_spec_expt.Software_label . _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details . save_ save_NMR_spec_expt__0_3 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_3 _NMR_spec_expt.Entry_ID 4377 _NMR_spec_expt.ID 3 _NMR_spec_expt.Name NOESY _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID . _NMR_spec_expt.NMR_spectrometer_label . _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID . _NMR_spec_expt.Software_label . _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details . save_ save_NMR_spec_expt__0_4 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_4 _NMR_spec_expt.Entry_ID 4377 _NMR_spec_expt.ID 4 _NMR_spec_expt.Name DQFC _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID . _NMR_spec_expt.NMR_spectrometer_label . _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID . _NMR_spec_expt.Software_label . _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details . save_ save_NMR_spec_expt__0_5 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_5 _NMR_spec_expt.Entry_ID 4377 _NMR_spec_expt.ID 5 _NMR_spec_expt.Name E.COSY _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID . _NMR_spec_expt.NMR_spectrometer_label . _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID . _NMR_spec_expt.Software_label . _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details . save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_set_one _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_set_one _Chem_shift_reference.Entry_ID 4377 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio_cit_ID _Chem_shift_ref.Indirect_shift_ratio_cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_cit_ID _Chem_shift_ref.Correction_val_cit_label _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 TSP 'methyl protons' . . . . ppm 0.00 internal direct . . . . . . . . . . 4377 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_set_one _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chemical_shifts_set_one _Assigned_chem_shift_list.Entry_ID 4377 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $experimental_conditions_one _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_set_one _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID . . 1 $sample_one . 4377 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 21 21 GLY H H 1 8.48 0.015 . 1 . . . . . . . . 4377 1 2 . 1 1 21 21 GLY HA2 H 1 3.98 0.015 . 1 . . . . . . . . 4377 1 3 . 1 1 21 21 GLY HA3 H 1 3.98 0.015 . 1 . . . . . . . . 4377 1 4 . 1 1 22 22 ASN H H 1 8.24 0.015 . 1 . . . . . . . . 4377 1 5 . 1 1 22 22 ASN HA H 1 4.75 0.015 . 1 . . . . . . . . 4377 1 6 . 1 1 22 22 ASN HB2 H 1 2.76 0.015 . 2 . . . . . . . . 4377 1 7 . 1 1 22 22 ASN HB3 H 1 2.85 0.015 . 2 . . . . . . . . 4377 1 8 . 1 1 23 23 GLN H H 1 8.10 0.015 . 1 . . . . . . . . 4377 1 9 . 1 1 23 23 GLN HA H 1 4.85 0.015 . 1 . . . . . . . . 4377 1 10 . 1 1 23 23 GLN HB2 H 1 1.92 0.015 . 1 . . . . . . . . 4377 1 11 . 1 1 23 23 GLN HB3 H 1 1.98 0.015 . 1 . . . . . . . . 4377 1 12 . 1 1 23 23 GLN HG2 H 1 2.24 0.015 . 2 . . . . . . . . 4377 1 13 . 1 1 23 23 GLN HG3 H 1 2.36 0.015 . 2 . . . . . . . . 4377 1 14 . 1 1 23 23 GLN HE21 H 1 6.78 0.015 . 2 . . . . . . . . 4377 1 15 . 1 1 23 23 GLN HE22 H 1 7.31 0.015 . 2 . . . . . . . . 4377 1 16 . 1 1 24 24 GLN H H 1 8.70 0.015 . 1 . . . . . . . . 4377 1 17 . 1 1 24 24 GLN HA H 1 4.73 0.015 . 1 . . . . . . . . 4377 1 18 . 1 1 24 24 GLN HB2 H 1 1.75 0.015 . 4 . . . . . . . . 4377 1 19 . 1 1 24 24 GLN HB3 H 1 2.10 0.015 . 4 . . . . . . . . 4377 1 20 . 1 1 24 24 GLN HG2 H 1 2.15 0.015 . 4 . . . . . . . . 4377 1 21 . 1 1 24 24 GLN HG3 H 1 2.15 0.015 . 4 . . . . . . . . 4377 1 22 . 1 1 24 24 GLN HE21 H 1 6.45 0.015 . 2 . . . . . . . . 4377 1 23 . 1 1 24 24 GLN HE22 H 1 7.14 0.015 . 2 . . . . . . . . 4377 1 24 . 1 1 25 25 CYS H H 1 9.73 0.015 . 1 . . . . . . . . 4377 1 25 . 1 1 25 25 CYS HA H 1 4.99 0.015 . 1 . . . . . . . . 4377 1 26 . 1 1 25 25 CYS HB2 H 1 3.07 0.015 . 1 . . . . . . . . 4377 1 27 . 1 1 25 25 CYS HB3 H 1 2.68 0.015 . 1 . . . . . . . . 4377 1 28 . 1 1 26 26 ASN H H 1 9.12 0.015 . 1 . . . . . . . . 4377 1 29 . 1 1 26 26 ASN HA H 1 4.77 0.015 . 1 . . . . . . . . 4377 1 30 . 1 1 26 26 ASN HB2 H 1 2.50 0.015 . 1 . . . . . . . . 4377 1 31 . 1 1 26 26 ASN HB3 H 1 3.46 0.015 . 1 . . . . . . . . 4377 1 32 . 1 1 26 26 ASN HD21 H 1 6.82 0.015 . 2 . . . . . . . . 4377 1 33 . 1 1 26 26 ASN HD22 H 1 7.30 0.015 . 2 . . . . . . . . 4377 1 34 . 1 1 27 27 TRP H H 1 9.22 0.015 . 1 . . . . . . . . 4377 1 35 . 1 1 27 27 TRP HA H 1 5.12 0.015 . 1 . . . . . . . . 4377 1 36 . 1 1 27 27 TRP HB2 H 1 2.70 0.015 . 1 . . . . . . . . 4377 1 37 . 1 1 27 27 TRP HB3 H 1 3.85 0.015 . 1 . . . . . . . . 4377 1 38 . 1 1 27 27 TRP HD1 H 1 7.21 0.015 . 1 . . . . . . . . 4377 1 39 . 1 1 27 27 TRP HE1 H 1 9.53 0.015 . 1 . . . . . . . . 4377 1 40 . 1 1 27 27 TRP HE3 H 1 7.57 0.015 . 1 . . . . . . . . 4377 1 41 . 1 1 27 27 TRP HZ2 H 1 7.46 0.015 . 1 . . . . . . . . 4377 1 42 . 1 1 27 27 TRP HZ3 H 1 6.90 0.015 . 1 . . . . . . . . 4377 1 43 . 1 1 27 27 TRP HH2 H 1 7.22 0.015 . 1 . . . . . . . . 4377 1 44 . 1 1 28 28 TYR H H 1 8.05 0.015 . 1 . . . . . . . . 4377 1 45 . 1 1 28 28 TYR HA H 1 1.20 0.015 . 1 . . . . . . . . 4377 1 46 . 1 1 28 28 TYR HB2 H 1 2.17 0.015 . 1 . . . . . . . . 4377 1 47 . 1 1 28 28 TYR HB3 H 1 2.72 0.015 . 1 . . . . . . . . 4377 1 48 . 1 1 28 28 TYR HD1 H 1 5.57 0.015 . 1 . . . . . . . . 4377 1 49 . 1 1 28 28 TYR HD2 H 1 5.57 0.015 . 1 . . . . . . . . 4377 1 50 . 1 1 28 28 TYR HE1 H 1 5.88 0.015 . 1 . . . . . . . . 4377 1 51 . 1 1 28 28 TYR HE2 H 1 5.88 0.015 . 1 . . . . . . . . 4377 1 52 . 1 1 29 29 GLY H H 1 7.15 0.015 . 1 . . . . . . . . 4377 1 53 . 1 1 29 29 GLY HA2 H 1 3.90 0.015 . 1 . . . . . . . . 4377 1 54 . 1 1 29 29 GLY HA3 H 1 3.90 0.015 . 1 . . . . . . . . 4377 1 55 . 1 1 30 30 THR H H 1 8.05 0.015 . 1 . . . . . . . . 4377 1 56 . 1 1 30 30 THR HA H 1 4.55 0.015 . 1 . . . . . . . . 4377 1 57 . 1 1 30 30 THR HB H 1 4.30 0.015 . 1 . . . . . . . . 4377 1 58 . 1 1 30 30 THR HG21 H 1 1.13 0.015 . 1 . . . . . . . . 4377 1 59 . 1 1 30 30 THR HG22 H 1 1.13 0.015 . 1 . . . . . . . . 4377 1 60 . 1 1 30 30 THR HG23 H 1 1.13 0.015 . 1 . . . . . . . . 4377 1 61 . 1 1 31 31 LEU H H 1 8.27 0.015 . 1 . . . . . . . . 4377 1 62 . 1 1 31 31 LEU HA H 1 5.29 0.015 . 1 . . . . . . . . 4377 1 63 . 1 1 31 31 LEU HB2 H 1 1.20 0.015 . 2 . . . . . . . . 4377 1 64 . 1 1 31 31 LEU HB3 H 1 1.63 0.015 . 2 . . . . . . . . 4377 1 65 . 1 1 31 31 LEU HG H 1 1.62 0.015 . 1 . . . . . . . . 4377 1 66 . 1 1 31 31 LEU HD11 H 1 0.70 0.015 . 2 . . . . . . . . 4377 1 67 . 1 1 31 31 LEU HD12 H 1 0.70 0.015 . 2 . . . . . . . . 4377 1 68 . 1 1 31 31 LEU HD13 H 1 0.70 0.015 . 2 . . . . . . . . 4377 1 69 . 1 1 31 31 LEU HD21 H 1 0.82 0.015 . 2 . . . . . . . . 4377 1 70 . 1 1 31 31 LEU HD22 H 1 0.82 0.015 . 2 . . . . . . . . 4377 1 71 . 1 1 31 31 LEU HD23 H 1 0.82 0.015 . 2 . . . . . . . . 4377 1 72 . 1 1 32 32 TYR H H 1 9.22 0.015 . 1 . . . . . . . . 4377 1 73 . 1 1 32 32 TYR HA H 1 4.86 0.015 . 1 . . . . . . . . 4377 1 74 . 1 1 32 32 TYR HB2 H 1 2.30 0.015 . 1 . . . . . . . . 4377 1 75 . 1 1 32 32 TYR HB3 H 1 2.92 0.015 . 1 . . . . . . . . 4377 1 76 . 1 1 32 32 TYR HD1 H 1 7.22 0.015 . 1 . . . . . . . . 4377 1 77 . 1 1 32 32 TYR HD2 H 1 7.22 0.015 . 1 . . . . . . . . 4377 1 78 . 1 1 32 32 TYR HE1 H 1 6.98 0.015 . 1 . . . . . . . . 4377 1 79 . 1 1 32 32 TYR HE2 H 1 6.98 0.015 . 1 . . . . . . . . 4377 1 80 . 1 1 33 33 PRO HA H 1 4.63 0.015 . 1 . . . . . . . . 4377 1 81 . 1 1 33 33 PRO HB2 H 1 1.75 0.015 . 2 . . . . . . . . 4377 1 82 . 1 1 33 33 PRO HB3 H 1 2.36 0.015 . 2 . . . . . . . . 4377 1 83 . 1 1 33 33 PRO HG2 H 1 1.93 0.015 . 2 . . . . . . . . 4377 1 84 . 1 1 33 33 PRO HG3 H 1 2.07 0.015 . 2 . . . . . . . . 4377 1 85 . 1 1 33 33 PRO HD2 H 1 3.86 0.015 . 2 . . . . . . . . 4377 1 86 . 1 1 33 33 PRO HD3 H 1 4.10 0.015 . 2 . . . . . . . . 4377 1 87 . 1 1 34 34 LEU H H 1 8.22 0.015 . 1 . . . . . . . . 4377 1 88 . 1 1 34 34 LEU HA H 1 5.27 0.015 . 1 . . . . . . . . 4377 1 89 . 1 1 34 34 LEU HB2 H 1 1.32 0.015 . 2 . . . . . . . . 4377 1 90 . 1 1 34 34 LEU HB3 H 1 1.60 0.015 . 2 . . . . . . . . 4377 1 91 . 1 1 34 34 LEU HG H 1 1.49 0.015 . 1 . . . . . . . . 4377 1 92 . 1 1 34 34 LEU HD11 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 93 . 1 1 34 34 LEU HD12 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 94 . 1 1 34 34 LEU HD13 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 95 . 1 1 34 34 LEU HD21 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 96 . 1 1 34 34 LEU HD22 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 97 . 1 1 34 34 LEU HD23 H 1 0.80 0.015 . 1 . . . . . . . . 4377 1 98 . 1 1 35 35 CYS H H 1 8.10 0.015 . 1 . . . . . . . . 4377 1 99 . 1 1 35 35 CYS HA H 1 4.56 0.015 . 1 . . . . . . . . 4377 1 100 . 1 1 35 35 CYS HB2 H 1 2.27 0.015 . 2 . . . . . . . . 4377 1 101 . 1 1 35 35 CYS HB3 H 1 3.72 0.015 . 2 . . . . . . . . 4377 1 102 . 1 1 36 36 VAL H H 1 10.79 0.015 . 1 . . . . . . . . 4377 1 103 . 1 1 36 36 VAL HA H 1 4.18 0.015 . 1 . . . . . . . . 4377 1 104 . 1 1 36 36 VAL HB H 1 2.10 0.015 . 1 . . . . . . . . 4377 1 105 . 1 1 36 36 VAL HG11 H 1 0.92 0.015 . 1 . . . . . . . . 4377 1 106 . 1 1 36 36 VAL HG12 H 1 0.92 0.015 . 1 . . . . . . . . 4377 1 107 . 1 1 36 36 VAL HG13 H 1 0.92 0.015 . 1 . . . . . . . . 4377 1 108 . 1 1 36 36 VAL HG21 H 1 0.98 0.015 . 1 . . . . . . . . 4377 1 109 . 1 1 36 36 VAL HG22 H 1 0.98 0.015 . 1 . . . . . . . . 4377 1 110 . 1 1 36 36 VAL HG23 H 1 0.98 0.015 . 1 . . . . . . . . 4377 1 111 . 1 1 37 37 THR H H 1 8.94 0.015 . 1 . . . . . . . . 4377 1 112 . 1 1 37 37 THR HA H 1 4.35 0.015 . 1 . . . . . . . . 4377 1 113 . 1 1 37 37 THR HB H 1 4.22 0.015 . 1 . . . . . . . . 4377 1 114 . 1 1 37 37 THR HG21 H 1 1.20 0.015 . 1 . . . . . . . . 4377 1 115 . 1 1 37 37 THR HG22 H 1 1.20 0.015 . 1 . . . . . . . . 4377 1 116 . 1 1 37 37 THR HG23 H 1 1.20 0.015 . 1 . . . . . . . . 4377 1 117 . 1 1 38 38 THR H H 1 7.69 0.015 . 1 . . . . . . . . 4377 1 118 . 1 1 38 38 THR HA H 1 4.27 0.015 . 1 . . . . . . . . 4377 1 119 . 1 1 38 38 THR HB H 1 3.81 0.015 . 1 . . . . . . . . 4377 1 120 . 1 1 38 38 THR HG21 H 1 1.02 0.015 . 1 . . . . . . . . 4377 1 121 . 1 1 38 38 THR HG22 H 1 1.02 0.015 . 1 . . . . . . . . 4377 1 122 . 1 1 38 38 THR HG23 H 1 1.02 0.015 . 1 . . . . . . . . 4377 1 123 . 1 1 39 39 THR H H 1 8.47 0.015 . 1 . . . . . . . . 4377 1 124 . 1 1 39 39 THR HA H 1 4.22 0.015 . 4 . . . . . . . . 4377 1 125 . 1 1 39 39 THR HB H 1 4.22 0.015 . 4 . . . . . . . . 4377 1 126 . 1 1 39 39 THR HG21 H 1 1.21 0.015 . 1 . . . . . . . . 4377 1 127 . 1 1 39 39 THR HG22 H 1 1.21 0.015 . 1 . . . . . . . . 4377 1 128 . 1 1 39 39 THR HG23 H 1 1.21 0.015 . 1 . . . . . . . . 4377 1 129 . 1 1 40 40 ASN H H 1 7.42 0.015 . 1 . . . . . . . . 4377 1 130 . 1 1 40 40 ASN HA H 1 4.52 0.015 . 1 . . . . . . . . 4377 1 131 . 1 1 40 40 ASN HB2 H 1 2.60 0.015 . 1 . . . . . . . . 4377 1 132 . 1 1 40 40 ASN HB3 H 1 2.60 0.015 . 1 . . . . . . . . 4377 1 133 . 1 1 41 41 GLY H H 1 8.43 0.015 . 1 . . . . . . . . 4377 1 134 . 1 1 41 41 GLY HA2 H 1 3.59 0.015 . 2 . . . . . . . . 4377 1 135 . 1 1 41 41 GLY HA3 H 1 4.12 0.015 . 2 . . . . . . . . 4377 1 136 . 1 1 42 42 TRP H H 1 8.14 0.015 . 1 . . . . . . . . 4377 1 137 . 1 1 42 42 TRP HA H 1 4.87 0.015 . 1 . . . . . . . . 4377 1 138 . 1 1 42 42 TRP HB2 H 1 2.86 0.015 . 1 . . . . . . . . 4377 1 139 . 1 1 42 42 TRP HB3 H 1 3.30 0.015 . 1 . . . . . . . . 4377 1 140 . 1 1 42 42 TRP HD1 H 1 6.99 0.015 . 1 . . . . . . . . 4377 1 141 . 1 1 42 42 TRP HE1 H 1 8.52 0.015 . 1 . . . . . . . . 4377 1 142 . 1 1 42 42 TRP HE3 H 1 7.24 0.015 . 1 . . . . . . . . 4377 1 143 . 1 1 42 42 TRP HZ2 H 1 5.62 0.015 . 1 . . . . . . . . 4377 1 144 . 1 1 42 42 TRP HZ3 H 1 6.02 0.015 . 1 . . . . . . . . 4377 1 145 . 1 1 42 42 TRP HH2 H 1 5.60 0.015 . 1 . . . . . . . . 4377 1 146 . 1 1 43 43 GLY H H 1 9.02 0.015 . 1 . . . . . . . . 4377 1 147 . 1 1 43 43 GLY HA2 H 1 3.80 0.015 . 2 . . . . . . . . 4377 1 148 . 1 1 43 43 GLY HA3 H 1 4.81 0.015 . 2 . . . . . . . . 4377 1 149 . 1 1 44 44 TRP H H 1 8.49 0.015 . 1 . . . . . . . . 4377 1 150 . 1 1 44 44 TRP HA H 1 5.26 0.015 . 1 . . . . . . . . 4377 1 151 . 1 1 44 44 TRP HB2 H 1 2.91 0.015 . 1 . . . . . . . . 4377 1 152 . 1 1 44 44 TRP HB3 H 1 3.25 0.015 . 1 . . . . . . . . 4377 1 153 . 1 1 44 44 TRP HD1 H 1 7.07 0.015 . 1 . . . . . . . . 4377 1 154 . 1 1 44 44 TRP HE1 H 1 9.75 0.015 . 1 . . . . . . . . 4377 1 155 . 1 1 44 44 TRP HE3 H 1 7.46 0.015 . 1 . . . . . . . . 4377 1 156 . 1 1 44 44 TRP HZ2 H 1 7.30 0.015 . 1 . . . . . . . . 4377 1 157 . 1 1 44 44 TRP HZ3 H 1 7.12 0.015 . 1 . . . . . . . . 4377 1 158 . 1 1 44 44 TRP HH2 H 1 7.20 0.015 . 1 . . . . . . . . 4377 1 159 . 1 1 45 45 GLU H H 1 8.20 0.015 . 1 . . . . . . . . 4377 1 160 . 1 1 45 45 GLU HA H 1 4.51 0.015 . 1 . . . . . . . . 4377 1 161 . 1 1 45 45 GLU HB2 H 1 1.58 0.015 . 2 . . . . . . . . 4377 1 162 . 1 1 45 45 GLU HB3 H 1 1.92 0.015 . 2 . . . . . . . . 4377 1 163 . 1 1 45 45 GLU HG2 H 1 2.20 0.015 . 2 . . . . . . . . 4377 1 164 . 1 1 45 45 GLU HG3 H 1 2.38 0.015 . 2 . . . . . . . . 4377 1 165 . 1 1 46 46 ASP H H 1 9.00 0.015 . 1 . . . . . . . . 4377 1 166 . 1 1 46 46 ASP HA H 1 4.15 0.015 . 1 . . . . . . . . 4377 1 167 . 1 1 46 46 ASP HB2 H 1 2.48 0.015 . 2 . . . . . . . . 4377 1 168 . 1 1 46 46 ASP HB3 H 1 2.86 0.015 . 2 . . . . . . . . 4377 1 169 . 1 1 47 47 GLN H H 1 8.15 0.015 . 1 . . . . . . . . 4377 1 170 . 1 1 47 47 GLN HA H 1 3.03 0.015 . 1 . . . . . . . . 4377 1 171 . 1 1 47 47 GLN HB2 H 1 2.15 0.015 . 4 . . . . . . . . 4377 1 172 . 1 1 47 47 GLN HB3 H 1 2.15 0.015 . 4 . . . . . . . . 4377 1 173 . 1 1 47 47 GLN HG2 H 1 2.28 0.015 . 4 . . . . . . . . 4377 1 174 . 1 1 47 47 GLN HG3 H 1 2.28 0.015 . 4 . . . . . . . . 4377 1 175 . 1 1 47 47 GLN HE21 H 1 6.71 0.015 . 2 . . . . . . . . 4377 1 176 . 1 1 47 47 GLN HE22 H 1 7.30 0.015 . 2 . . . . . . . . 4377 1 177 . 1 1 48 48 ARG H H 1 6.72 0.015 . 1 . . . . . . . . 4377 1 178 . 1 1 48 48 ARG HA H 1 4.04 0.015 . 1 . . . . . . . . 4377 1 179 . 1 1 48 48 ARG HB2 H 1 1.76 0.015 . 1 . . . . . . . . 4377 1 180 . 1 1 48 48 ARG HB3 H 1 1.76 0.015 . 1 . . . . . . . . 4377 1 181 . 1 1 48 48 ARG HG2 H 1 1.41 0.015 . 2 . . . . . . . . 4377 1 182 . 1 1 48 48 ARG HG3 H 1 1.58 0.015 . 2 . . . . . . . . 4377 1 183 . 1 1 48 48 ARG HD2 H 1 3.05 0.015 . 2 . . . . . . . . 4377 1 184 . 1 1 48 48 ARG HD3 H 1 3.15 0.015 . 2 . . . . . . . . 4377 1 185 . 1 1 48 48 ARG HE H 1 7.20 0.015 . 1 . . . . . . . . 4377 1 186 . 1 1 49 49 SER H H 1 8.03 0.015 . 1 . . . . . . . . 4377 1 187 . 1 1 49 49 SER HA H 1 5.07 0.015 . 1 . . . . . . . . 4377 1 188 . 1 1 49 49 SER HB2 H 1 4.10 0.015 . 1 . . . . . . . . 4377 1 189 . 1 1 49 49 SER HB3 H 1 4.03 0.015 . 1 . . . . . . . . 4377 1 190 . 1 1 50 50 CYS H H 1 8.55 0.015 . 1 . . . . . . . . 4377 1 191 . 1 1 50 50 CYS HA H 1 5.11 0.015 . 1 . . . . . . . . 4377 1 192 . 1 1 50 50 CYS HB2 H 1 2.90 0.015 . 1 . . . . . . . . 4377 1 193 . 1 1 50 50 CYS HB3 H 1 2.80 0.015 . 1 . . . . . . . . 4377 1 194 . 1 1 51 51 ILE H H 1 8.30 0.015 . 1 . . . . . . . . 4377 1 195 . 1 1 51 51 ILE HA H 1 3.82 0.015 . 1 . . . . . . . . 4377 1 196 . 1 1 51 51 ILE HB H 1 0.34 0.015 . 1 . . . . . . . . 4377 1 197 . 1 1 51 51 ILE HG12 H 1 -0.95 0.015 . 2 . . . . . . . . 4377 1 198 . 1 1 51 51 ILE HG13 H 1 0.00 0.015 . 2 . . . . . . . . 4377 1 199 . 1 1 51 51 ILE HG21 H 1 0.30 0.015 . 1 . . . . . . . . 4377 1 200 . 1 1 51 51 ILE HG22 H 1 0.30 0.015 . 1 . . . . . . . . 4377 1 201 . 1 1 51 51 ILE HG23 H 1 0.30 0.015 . 1 . . . . . . . . 4377 1 202 . 1 1 51 51 ILE HD11 H 1 -0.75 0.015 . 1 . . . . . . . . 4377 1 203 . 1 1 51 51 ILE HD12 H 1 -0.75 0.015 . 1 . . . . . . . . 4377 1 204 . 1 1 51 51 ILE HD13 H 1 -0.75 0.015 . 1 . . . . . . . . 4377 1 205 . 1 1 52 52 ALA H H 1 8.17 0.015 . 1 . . . . . . . . 4377 1 206 . 1 1 52 52 ALA HA H 1 4.03 0.015 . 1 . . . . . . . . 4377 1 207 . 1 1 52 52 ALA HB1 H 1 1.11 0.015 . 1 . . . . . . . . 4377 1 208 . 1 1 52 52 ALA HB2 H 1 1.11 0.015 . 1 . . . . . . . . 4377 1 209 . 1 1 52 52 ALA HB3 H 1 1.11 0.015 . 1 . . . . . . . . 4377 1 210 . 1 1 53 53 ARG H H 1 8.89 0.015 . 1 . . . . . . . . 4377 1 211 . 1 1 53 53 ARG HA H 1 3.70 0.015 . 1 . . . . . . . . 4377 1 212 . 1 1 53 53 ARG HB2 H 1 1.86 0.015 . 1 . . . . . . . . 4377 1 213 . 1 1 53 53 ARG HB3 H 1 1.86 0.015 . 1 . . . . . . . . 4377 1 214 . 1 1 53 53 ARG HG2 H 1 1.58 0.015 . 2 . . . . . . . . 4377 1 215 . 1 1 53 53 ARG HG3 H 1 1.69 0.015 . 2 . . . . . . . . 4377 1 216 . 1 1 53 53 ARG HD2 H 1 3.27 0.015 . 1 . . . . . . . . 4377 1 217 . 1 1 53 53 ARG HD3 H 1 3.27 0.015 . 1 . . . . . . . . 4377 1 218 . 1 1 53 53 ARG HE H 1 7.41 0.015 . 1 . . . . . . . . 4377 1 219 . 1 1 54 54 SER H H 1 8.94 0.015 . 1 . . . . . . . . 4377 1 220 . 1 1 54 54 SER HA H 1 4.07 0.015 . 1 . . . . . . . . 4377 1 221 . 1 1 54 54 SER HB2 H 1 3.94 0.015 . 2 . . . . . . . . 4377 1 222 . 1 1 54 54 SER HB3 H 1 4.00 0.015 . 2 . . . . . . . . 4377 1 223 . 1 1 55 55 THR H H 1 6.82 0.015 . 1 . . . . . . . . 4377 1 224 . 1 1 55 55 THR HA H 1 4.07 0.015 . 1 . . . . . . . . 4377 1 225 . 1 1 55 55 THR HB H 1 3.75 0.015 . 1 . . . . . . . . 4377 1 226 . 1 1 55 55 THR HG21 H 1 1.22 0.015 . 1 . . . . . . . . 4377 1 227 . 1 1 55 55 THR HG22 H 1 1.22 0.015 . 1 . . . . . . . . 4377 1 228 . 1 1 55 55 THR HG23 H 1 1.22 0.015 . 1 . . . . . . . . 4377 1 229 . 1 1 56 56 CYS H H 1 8.49 0.015 . 1 . . . . . . . . 4377 1 230 . 1 1 56 56 CYS HA H 1 4.26 0.015 . 1 . . . . . . . . 4377 1 231 . 1 1 56 56 CYS HB2 H 1 2.69 0.015 . 1 . . . . . . . . 4377 1 232 . 1 1 56 56 CYS HB3 H 1 3.32 0.015 . 1 . . . . . . . . 4377 1 233 . 1 1 57 57 ALA H H 1 8.28 0.015 . 1 . . . . . . . . 4377 1 234 . 1 1 57 57 ALA HA H 1 4.36 0.015 . 1 . . . . . . . . 4377 1 235 . 1 1 57 57 ALA HB1 H 1 1.50 0.015 . 1 . . . . . . . . 4377 1 236 . 1 1 57 57 ALA HB2 H 1 1.50 0.015 . 1 . . . . . . . . 4377 1 237 . 1 1 57 57 ALA HB3 H 1 1.50 0.015 . 1 . . . . . . . . 4377 1 238 . 1 1 58 58 ALA H H 1 7.17 0.015 . 1 . . . . . . . . 4377 1 239 . 1 1 58 58 ALA HA H 1 4.48 0.015 . 1 . . . . . . . . 4377 1 240 . 1 1 58 58 ALA HB1 H 1 1.60 0.015 . 1 . . . . . . . . 4377 1 241 . 1 1 58 58 ALA HB2 H 1 1.60 0.015 . 1 . . . . . . . . 4377 1 242 . 1 1 58 58 ALA HB3 H 1 1.60 0.015 . 1 . . . . . . . . 4377 1 243 . 1 1 59 59 GLN H H 1 7.10 0.015 . 1 . . . . . . . . 4377 1 244 . 1 1 59 59 GLN HA H 1 4.25 0.015 . 1 . . . . . . . . 4377 1 245 . 1 1 59 59 GLN HB2 H 1 1.42 0.015 . 2 . . . . . . . . 4377 1 246 . 1 1 59 59 GLN HB3 H 1 1.52 0.015 . 2 . . . . . . . . 4377 1 247 . 1 1 59 59 GLN HG2 H 1 1.95 0.015 . 2 . . . . . . . . 4377 1 248 . 1 1 59 59 GLN HG3 H 1 2.56 0.015 . 2 . . . . . . . . 4377 1 249 . 1 1 59 59 GLN HE21 H 1 7.25 0.015 . 2 . . . . . . . . 4377 1 250 . 1 1 59 59 GLN HE22 H 1 7.92 0.015 . 2 . . . . . . . . 4377 1 251 . 1 1 60 60 PRO HA H 1 4.58 0.015 . 1 . . . . . . . . 4377 1 252 . 1 1 60 60 PRO HB2 H 1 2.10 0.015 . 2 . . . . . . . . 4377 1 253 . 1 1 60 60 PRO HB3 H 1 2.30 0.015 . 2 . . . . . . . . 4377 1 254 . 1 1 60 60 PRO HG2 H 1 2.22 0.015 . 1 . . . . . . . . 4377 1 255 . 1 1 60 60 PRO HG3 H 1 2.22 0.015 . 1 . . . . . . . . 4377 1 256 . 1 1 60 60 PRO HD2 H 1 3.58 0.015 . 2 . . . . . . . . 4377 1 257 . 1 1 60 60 PRO HD3 H 1 3.80 0.015 . 2 . . . . . . . . 4377 1 258 . 1 1 61 61 ALA H H 1 7.95 0.015 . 1 . . . . . . . . 4377 1 259 . 1 1 61 61 ALA HA H 1 4.22 0.015 . 1 . . . . . . . . 4377 1 260 . 1 1 61 61 ALA HB1 H 1 1.25 0.015 . 1 . . . . . . . . 4377 1 261 . 1 1 61 61 ALA HB2 H 1 1.25 0.015 . 1 . . . . . . . . 4377 1 262 . 1 1 61 61 ALA HB3 H 1 1.25 0.015 . 1 . . . . . . . . 4377 1 263 . 1 1 62 62 PRO HA H 1 4.38 0.015 . 1 . . . . . . . . 4377 1 264 . 1 1 62 62 PRO HB2 H 1 1.76 0.015 . 2 . . . . . . . . 4377 1 265 . 1 1 62 62 PRO HB3 H 1 2.15 0.015 . 2 . . . . . . . . 4377 1 266 . 1 1 62 62 PRO HG2 H 1 0.52 0.015 . 2 . . . . . . . . 4377 1 267 . 1 1 62 62 PRO HG3 H 1 1.52 0.015 . 2 . . . . . . . . 4377 1 268 . 1 1 62 62 PRO HD2 H 1 3.02 0.015 . 1 . . . . . . . . 4377 1 269 . 1 1 62 62 PRO HD3 H 1 3.02 0.015 . 1 . . . . . . . . 4377 1 270 . 1 1 63 63 PHE H H 1 7.89 0.015 . 1 . . . . . . . . 4377 1 271 . 1 1 63 63 PHE HA H 1 4.52 0.015 . 1 . . . . . . . . 4377 1 272 . 1 1 63 63 PHE HB2 H 1 2.38 0.015 . 2 . . . . . . . . 4377 1 273 . 1 1 63 63 PHE HB3 H 1 3.60 0.015 . 2 . . . . . . . . 4377 1 274 . 1 1 63 63 PHE HD1 H 1 6.84 0.015 . 1 . . . . . . . . 4377 1 275 . 1 1 63 63 PHE HD2 H 1 6.84 0.015 . 1 . . . . . . . . 4377 1 276 . 1 1 63 63 PHE HE1 H 1 7.22 0.015 . 1 . . . . . . . . 4377 1 277 . 1 1 63 63 PHE HE2 H 1 7.22 0.015 . 1 . . . . . . . . 4377 1 278 . 1 1 63 63 PHE HZ H 1 7.16 0.015 . 1 . . . . . . . . 4377 1 279 . 1 1 64 64 GLY H H 1 7.93 0.015 . 1 . . . . . . . . 4377 1 280 . 1 1 64 64 GLY HA2 H 1 3.78 0.015 . 2 . . . . . . . . 4377 1 281 . 1 1 64 64 GLY HA3 H 1 4.71 0.015 . 2 . . . . . . . . 4377 1 282 . 1 1 65 65 ILE H H 1 8.66 0.015 . 1 . . . . . . . . 4377 1 283 . 1 1 65 65 ILE HA H 1 4.29 0.015 . 1 . . . . . . . . 4377 1 284 . 1 1 65 65 ILE HB H 1 1.75 0.015 . 1 . . . . . . . . 4377 1 285 . 1 1 65 65 ILE HG12 H 1 0.99 0.015 . 2 . . . . . . . . 4377 1 286 . 1 1 65 65 ILE HG13 H 1 1.09 0.015 . 2 . . . . . . . . 4377 1 287 . 1 1 65 65 ILE HG21 H 1 0.90 0.015 . 1 . . . . . . . . 4377 1 288 . 1 1 65 65 ILE HG22 H 1 0.90 0.015 . 1 . . . . . . . . 4377 1 289 . 1 1 65 65 ILE HG23 H 1 0.90 0.015 . 1 . . . . . . . . 4377 1 290 . 1 1 65 65 ILE HD11 H 1 1.57 0.015 . 1 . . . . . . . . 4377 1 291 . 1 1 65 65 ILE HD12 H 1 1.57 0.015 . 1 . . . . . . . . 4377 1 292 . 1 1 65 65 ILE HD13 H 1 1.57 0.015 . 1 . . . . . . . . 4377 1 293 . 1 1 66 66 VAL H H 1 8.81 0.015 . 1 . . . . . . . . 4377 1 294 . 1 1 66 66 VAL HA H 1 4.56 0.015 . 1 . . . . . . . . 4377 1 295 . 1 1 66 66 VAL HB H 1 2.14 0.015 . 1 . . . . . . . . 4377 1 296 . 1 1 66 66 VAL HG11 H 1 0.97 0.015 . 1 . . . . . . . . 4377 1 297 . 1 1 66 66 VAL HG12 H 1 0.97 0.015 . 1 . . . . . . . . 4377 1 298 . 1 1 66 66 VAL HG13 H 1 0.97 0.015 . 1 . . . . . . . . 4377 1 299 . 1 1 66 66 VAL HG21 H 1 0.85 0.015 . 1 . . . . . . . . 4377 1 300 . 1 1 66 66 VAL HG22 H 1 0.85 0.015 . 1 . . . . . . . . 4377 1 301 . 1 1 66 66 VAL HG23 H 1 0.85 0.015 . 1 . . . . . . . . 4377 1 302 . 1 1 67 67 GLY H H 1 8.35 0.015 . 1 . . . . . . . . 4377 1 303 . 1 1 67 67 GLY HA2 H 1 4.15 0.015 . 1 . . . . . . . . 4377 1 304 . 1 1 67 67 GLY HA3 H 1 4.15 0.015 . 1 . . . . . . . . 4377 1 305 . 1 1 68 68 SER H H 1 8.19 0.015 . 1 . . . . . . . . 4377 1 306 . 1 1 68 68 SER HA H 1 4.49 0.015 . 1 . . . . . . . . 4377 1 307 . 1 1 68 68 SER HB2 H 1 3.82 0.015 . 2 . . . . . . . . 4377 1 308 . 1 1 68 68 SER HB3 H 1 3.88 0.015 . 2 . . . . . . . . 4377 1 stop_ save_