data_5894 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 5894 _Entry.Title ; 1H, 13C, and 15N resonance assignments for the N-terminal domain of Drosophila Stem-Loop Binding Protein ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2003-07-31 _Entry.Accession_date 2003-08-01 _Entry.Last_release_date 2004-10-25 _Entry.Original_release_date 2004-10-25 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 2.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype . _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Roopa Thapar . . . 5894 2 William Marzluff . F. . 5894 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 5894 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '13C chemical shifts' 280 5894 '15N chemical shifts' 82 5894 '1H chemical shifts' 145 5894 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2004-10-25 2003-07-31 original author . 5894 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 5894 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 15260482 _Citation.Full_citation . _Citation.Title ; The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structure. ; _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Biochemistry _Citation.Journal_name_full . _Citation.Journal_volume 43 _Citation.Journal_issue 29 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 9390 _Citation.Page_last 9400 _Citation.Year 2004 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Roopa Thapar . . . 5894 1 2 G. Mueller . A. . 5894 1 3 William Marzluff . F. . 5894 1 stop_ loop_ _Citation_keyword.Keyword _Citation_keyword.Entry_ID _Citation_keyword.Citation_ID 'histone mRNA' 5894 1 'NMR assignment' 5894 1 SLBP 5894 1 translation 5894 1 stop_ save_ save_ref_1 _Citation.Sf_category citations _Citation.Sf_framecode ref_1 _Citation.Entry_ID 5894 _Citation.ID 2 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 12189204 _Citation.Full_citation ; Battle DJ, Doudna JA. Specificity of RNA-RNA helix recognition. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11676-81. Epub 2002 Aug 20. ; _Citation.Title 'Specificity of RNA-RNA helix recognition.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Proc. Natl. Acad. Sci. U.S.A.' _Citation.Journal_name_full 'Proceedings of the National Academy of Sciences of the United States of America' _Citation.Journal_volume 99 _Citation.Journal_issue 18 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0027-8424 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 11676 _Citation.Page_last 11681 _Citation.Year 2002 _Citation.Details ; Functional RNAs often form compact structures characterized by closely packed helices. Crystallographic analysis of several large RNAs revealed a prevalent interaction in which unpaired adenosine residues dock into the minor groove of a receptor helix. This A-minor motif, potentially the most important element responsible for global RNA architecture, has also been suggested to contribute to the fidelity of protein synthesis by discriminating against near-cognate tRNAs on the ribosome. The specificity of A-minor interactions is fundamental to RNA tertiary structure formation, as well as to their proposed role in translational accuracy. To investigate A-minor motif specificity, we analyzed mutations in an A-minor interaction within the Tetrahymena group I self-splicing intron. Thermodynamic and x-ray crystallographic results show that the A-minor interaction strongly prefers canonical base pairs over base mismatches in the receptor helix, enabling RNA interhelical packing through specific recognition of Watson-Crick minor groove geometry. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 'Daniel J.' Battle D. J. . 5894 2 2 'Jennifer A.' Doudna J. A. . 5894 2 stop_ save_ save_ref_2 _Citation.Sf_category citations _Citation.Sf_framecode ref_2 _Citation.Entry_ID 5894 _Citation.ID 3 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 10571029 _Citation.Full_citation ; Dominski Z, Marzluff WF. Formation of the 3' end of histone mRNA. Gene. 1999 Oct 18;239(1):1-14. Review. ; _Citation.Title "Formation of the 3' end of histone mRNA." _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Gene _Citation.Journal_name_full Gene _Citation.Journal_volume 239 _Citation.Journal_issue 1 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0378-1119 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1 _Citation.Page_last 14 _Citation.Year 1999 _Citation.Details ; All metazoan messenger RNAs, with the exception of the replication-dependent histone mRNAs, terminate at the 3' end with a poly(A) tail. Replication-dependent histone mRNAs end instead in a conserved 26-nucleotide sequence that contains a 16-nucleotide stem-loop. Formation of the 3' end of histone mRNA occurs by endonucleolytic cleavage of pre-mRNA releasing the mature mRNA from the chromatin template. Cleavage requires several trans-acting factors, including a protein, the stem-loop binding protein (SLBP), which binds the 26-nucleotide sequence; and a small nuclear RNP, U7 snRNP. There are probably additional factors also required for cleavage. One of the functions of the SLBP is to stabilize binding of the U7 snRNP to the histone pre-mRNA. In the nucleus, both U7 snRNP and SLBP are present in coiled bodies, structures that are associated with histone genes and may play a direct role in histone pre-mRNA processing in vivo. One of the major regulatory events in the cell cycle is regulation of histone pre-mRNA processing, which is at least partially mediated by cell-cycle regulation of the levels of the SLBP protein. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Z. Dominski Z. . . 5894 3 2 'W. F.' Marzluff W. F. . 5894 3 stop_ save_ save_ref_3 _Citation.Sf_category citations _Citation.Sf_framecode ref_3 _Citation.Entry_ID 5894 _Citation.ID 4 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 12192062 _Citation.Full_citation ; Dominski Z, Yang XC, Raska CS, Santiago C, Borchers CH, Duronio RJ, Marzluff WF. 3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system. Mol Cell Biol. 2002 Sep;22(18):6648-60. ; _Citation.Title "3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system." _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Mol. Cell. Biol.' _Citation.Journal_name_full 'Molecular and cellular biology' _Citation.Journal_volume 22 _Citation.Journal_issue 18 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0270-7306 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 6648 _Citation.Page_last 6660 _Citation.Year 2002 _Citation.Details ; Synthetic pre-mRNAs containing the processing signals encoded by Drosophila melanogaster histone genes undergo efficient and faithful endonucleolytic cleavage in nuclear extracts prepared from Drosophila cultured cells and 0- to 13-h-old embryos. Biochemical requirements for the in vitro cleavage are similar to those previously described for the 3' end processing of mammalian histone pre-mRNAs. Drosophila 3' end processing does not require ATP and occurs in the presence of EDTA. However, in contrast to mammalian processing, Drosophila processing generates the final product ending four nucleotides after the stem-loop. Cleavage of the Drosophila substrates is abolished by depleting the extract of the Drosophila stem-loop binding protein (dSLBP), indicating that both dSLBP and the stem-loop structure in histone pre-mRNA are essential components of the processing machinery. Recombinant dSLBP expressed in insect cells by using the baculovirus system efficiently complements the depleted extract. Only the RNA-binding domain plus the 17 amino acids at the C terminus of dSLBP are required for processing. The full-length dSLBP expressed in insect cells is quantitatively phosphorylated on four residues in the C-terminal region. Dephosphorylation of the recombinant dSLBP reduces processing activity. Human and Drosophila SLBPs are not interchangeable and strongly inhibit processing in the heterologous extracts. The RNA-binding domain of the dSLBP does not substitute for the RNA-binding domain of the human SLBP in histone pre-mRNA processing in mammalian extracts. In addition to the stem-loop structure and dSLBP, 3' processing in Drosophila nuclear extracts depends on the presence of a short stretch of purines located ca. 20 nucleotides downstream from the stem, and an Sm-reactive factor, most likely the Drosophila counterpart of vertebrate U7 snRNP. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Zbigniew Dominski Z. . . 5894 4 2 Xiao-Cui Yang X. C. . 5894 4 3 'Christy S.' Raska C. S. . 5894 4 4 Carlos Santiago C. . . 5894 4 5 'Christoph H.' Borchers C. H. . 5894 4 6 'Robert J.' Duronio R. J. . 5894 4 7 'William F.' Marzluff W. F. . 5894 4 stop_ save_ save_ref_4 _Citation.Sf_category citations _Citation.Sf_framecode ref_4 _Citation.Entry_ID 5894 _Citation.ID 5 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID . _Citation.Full_citation ; Grzesiek, S. and Bax, A. (1992) J. Magn. Reson. 99, 201-207. ; _Citation.Title . _Citation.Status . _Citation.Type . _Citation.Journal_abbrev . _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . save_ save_ref_5 _Citation.Sf_category citations _Citation.Sf_framecode ref_5 _Citation.Entry_ID 5894 _Citation.ID 6 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID . _Citation.Full_citation ; Grzesiek, S., Anglister, J. and Bax, A. (1993) J. Magn. Reson. B 101, 114-119. ; _Citation.Title . _Citation.Status . _Citation.Type . _Citation.Journal_abbrev . _Citation.Journal_name_full . _Citation.Journal_volume . _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first . _Citation.Page_last . _Citation.Year . _Citation.Details . save_ save_ref_6 _Citation.Sf_category citations _Citation.Sf_framecode ref_6 _Citation.Entry_ID 5894 _Citation.ID 7 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 12473341 _Citation.Full_citation ; Marzluff WF, Duronio RJ. Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequences. Curr Opin Cell Biol. 2002 Dec;14(6):692-9. Review. ; _Citation.Title 'Histone mRNA expression: multiple levels of cell cycle regulation and important developmental consequences.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Curr. Opin. Cell Biol.' _Citation.Journal_name_full 'Current opinion in cell biology' _Citation.Journal_volume 14 _Citation.Journal_issue 6 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0955-0674 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 692 _Citation.Page_last 699 _Citation.Year 2002 _Citation.Details ; Histone mRNA metabolism is tightly coupled to cell cycle progression and to rates of DNA synthesis. The recent identification of several novel proteins involved in histone gene transcription and pre-mRNA processing has shed light on the variety of mechanisms cells employ to achieve this coupling. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 'William F.' Marzluff W. F. . 5894 7 2 'Robert J.' Duronio R. J. . 5894 7 stop_ save_ save_ref_7 _Citation.Sf_category citations _Citation.Sf_framecode ref_7 _Citation.Entry_ID 5894 _Citation.ID 8 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 12242288 _Citation.Full_citation ; Sanchez R, Marzluff WF. The stem-loop binding protein is required for efficient translation of histone mRNA in vivo and in vitro. Mol Cell Biol. 2002 Oct;22(20):7093-104. ; _Citation.Title 'The stem-loop binding protein is required for efficient translation of histone mRNA in vivo and in vitro.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Mol. Cell. Biol.' _Citation.Journal_name_full 'Molecular and cellular biology' _Citation.Journal_volume 22 _Citation.Journal_issue 20 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0270-7306 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 7093 _Citation.Page_last 7104 _Citation.Year 2002 _Citation.Details ; Metazoan replication-dependent histone mRNAs end in a conserved stem-loop rather than in the poly(A) tail found on all other mRNAs. The 3' end of histone mRNA binds a single class of proteins, the stem-loop binding proteins (SLBP). In Xenopus, there are two SLBPs: xSLBP1, the homologue of the mammalian SLBP, which is required for processing of histone pre-mRNA, and xSLBP2, which is expressed only during oogenesis and is bound to the stored histone mRNA in Xenopus oocytes. The stem-loop is required for efficient translation of histone mRNAs and substitutes for the poly(A) tail, which is required for efficient translation of other eucaryotic mRNAs. When a rabbit reticulocyte lysate is programmed with uncapped luciferase mRNA ending in the histone stem-loop, there is a three- to sixfold increase in translation in the presence of xSLBP1 while xSLBP2 has no effect on translation. Neither SLBP affected the translation of a luciferase mRNA ending in a mutant stem-loop that does not bind SLBP. Capped luciferase mRNAs ending in the stem-loop were injected into Xenopus oocytes after overexpression of either xSLBP1 or xSLBP2. Overexpression of xSLBP1 in the oocytes stimulated translation, while overexpression of xSLBP2 reduced translation of the luciferase mRNA ending in the histone stem-loop. A small region in the N-terminal portion of xSLBP1 is required to stimulate translation both in vivo and in vitro. An MS2-human SLBP1 fusion protein can activate translation of a reporter mRNA ending in an MS2 binding site, indicating that xSLBP1 only needs to be recruited to the 3' end of the mRNA but does not need to be directly bound to the histone stem-loop to activate translation. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Ricardo Sanchez R. . . 5894 8 2 'William F.' Marzluff W. F. . 5894 8 stop_ save_ save_ref_8 _Citation.Sf_category citations _Citation.Sf_framecode ref_8 _Citation.Entry_ID 5894 _Citation.ID 9 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 11157774 _Citation.Full_citation ; Sullivan E, Santiago C, Parker ED, Dominski Z, Yang X, Lanzotti DJ, Ingledue TC, Marzluff WF, Duronio RJ. Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression. Genes Dev. 2001 Jan 15;15(2):173-87. ; _Citation.Title 'Drosophila stem loop binding protein coordinates accumulation of mature histone mRNA with cell cycle progression.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Genes Dev.' _Citation.Journal_name_full 'Genes & development' _Citation.Journal_volume 15 _Citation.Journal_issue 2 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0890-9369 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 173 _Citation.Page_last 187 _Citation.Year 2001 _Citation.Details ; Replication-associated histone genes encode the only metazoan mRNAs that lack polyA tails, ending instead in a conserved 26-nt sequence that forms a stem-loop. Most of the regulation of mammalian histone mRNA is posttranscriptional and mediated by this unique 3' end. Stem-loop-binding protein (SLBP) binds to the histone mRNA 3' end and is thought to participate in all aspects of histone mRNA metabolism, including cell cycle regulation. To examine SLBP function genetically, we have cloned the gene encoding Drosophila SLBP (dSLBP) by a yeast three-hybrid method and have isolated mutations in dSLBP. dSLBP function is required both zygotically and maternally. Strong dSLBP alleles cause zygotic lethality late in development and result in production of stable histone mRNA that accumulates in nonreplicating cells. These histone mRNAs are cytoplasmic and have polyadenylated 3' ends like other polymerase II transcripts. Hypomorphic dSLBP alleles support zygotic development but cause female sterility. Eggs from these females contain dramatically reduced levels of histone mRNA, and mutant embryos are not able to complete the syncytial embryonic cycles. This is in part because of a failure of chromosome condensation at mitosis that blocks normal anaphase. These data demonstrate that dSLBP is required in vivo for 3' end processing of histone pre-mRNA, and that this is an essential function for development. Moreover, dSLBP-dependent processing plays an important role in coupling histone mRNA production with the cell cycle. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 E. Sullivan E. . . 5894 9 2 C. Santiago C. . . 5894 9 3 'E. D.' Parker E. D. . 5894 9 4 Z. Dominski Z. . . 5894 9 5 X. Yang X. . . 5894 9 6 'D. J.' Lanzotti D. J. . 5894 9 7 'T. C.' Ingledue T. C. . 5894 9 8 'W. F.' Marzluff W. F. . 5894 9 9 'R. J.' Duronio R. J. . 5894 9 stop_ save_ save_ref_9 _Citation.Sf_category citations _Citation.Sf_framecode ref_9 _Citation.Entry_ID 5894 _Citation.ID 10 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 7899087 _Citation.Full_citation ; Williams AS, Marzluff WF. The sequence of the stem and flanking sequences at the 3' end of histone mRNA are critical determinants for the binding of the stem-loop binding protein. Nucleic Acids Res. 1995 Feb 25;23(4):654-62. ; _Citation.Title "The sequence of the stem and flanking sequences at the 3' end of histone mRNA are critical determinants for the binding of the stem-loop binding protein." _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Nucleic Acids Res.' _Citation.Journal_name_full 'Nucleic acids research' _Citation.Journal_volume 23 _Citation.Journal_issue 4 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0305-1048 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 654 _Citation.Page_last 662 _Citation.Year 1995 _Citation.Details ; Complexes of different electrophoretic mobility containing the stem-loop binding protein, a 45 kDa protein, bound to the stem-loop at the 3' end of histone mRNA, are present in both nuclear and cytoplasmic extracts from mammalian cells. We have determined the effect of changes in the loop, in the stem and in the flanking sequences on the affinity of the SLBP for the 3' end of histone mRNA. The sequence of the stem is particularly critical for SLBP binding. Specific sequences both 5' and 3' of the stem-loop are also required for high-affinity binding. Expanding the four base loop by one or two uridines reduced but did not abolish SLBP binding. RNA footprinting experiments show that the flanking sequences on both sides of the stem-loop are critical for efficient binding, but that cleavages in the loop do not abolish binding. Thus all three regions of the RNA sequence contribute to SLBP binding, suggesting that the 26 nt at the 3' end of histone mRNA forms a defined tertiary structure recognized by the SLBP. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 'A. S.' Williams A. S. . 5894 10 2 'W. F.' Marzluff W. F. . 5894 10 stop_ save_ save_ref_10 _Citation.Sf_category citations _Citation.Sf_framecode ref_10 _Citation.Entry_ID 5894 _Citation.ID 11 _Citation.Class 'reference citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 1737021 _Citation.Full_citation ; Wishart DS, Sykes BD, Richards FM. The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry. 1992 Feb 18;31(6):1647-51. ; _Citation.Title 'The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev Biochemistry _Citation.Journal_name_full Biochemistry _Citation.Journal_volume 31 _Citation.Journal_issue 6 _Citation.Journal_ASTM . _Citation.Journal_ISSN 0006-2960 _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 1647 _Citation.Page_last 1651 _Citation.Year 1992 _Citation.Details ; Previous studies by Wishart et al. [Wishart, D. S., Sykes, B. D., & Richards, F. M. (1991) J. Mol. Biol. (in press)] have demonstrated that 1H NMR chemical shifts are strongly dependent on the character and nature of protein secondary structure. In particular, it has been found that the 1H NMR chemical shift of the alpha-CH proton of all 20 naturally occurring amino acids experiences an upfield shift (with respect to the random coil value) when in a helical configuration and a comparable downfield shift when in a beta-strand extended configuration. On the basis of these observations, a technique is described for rapidly and quantitatively determining the identity, extent, and location of secondary structural elements in proteins based on the simple inspection of the alpha-CH 1H resonance assignments. A number of examples are provided to demonstrate both the simplicity and the accuracy of the technique. This new method is found to be almost as accurate as the more traditional NOE-based methods of determining secondary structure and could prove to be particularly useful in light of the recent development of sequential assignment techniques which are now almost NOE-independent [Ikura, M., Kay, L. E., & Bax, A. (1990) Biochemistry 29, 4659-4667]. We suggest that this new procedure should not necessarily be seen as a substitute to existing rigorous methods for secondary structure determination but, rather, should be viewed as a complement to these approaches. ; loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 'D. S.' Wishart D. S. . 5894 11 2 'B. D.' Sykes B. D. . 5894 11 3 'F. M.' Richards F. M. . 5894 11 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_system_SLBP _Assembly.Sf_category assembly _Assembly.Sf_framecode system_SLBP _Assembly.Entry_ID 5894 _Assembly.ID 1 _Assembly.Name 'drosophila SLBP N-terminus' _Assembly.BMRB_code . _Assembly.Number_of_components . _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state 'all free' _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Assembly_type.Type _Assembly_type.Entry_ID _Assembly_type.Assembly_ID monomer 5894 1 stop_ loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'Drosophila SLBP N-terminus' 1 $dSLBP_N-term . . . native . . . . . 5894 1 stop_ loop_ _Assembly_common_name.Name _Assembly_common_name.Type _Assembly_common_name.Entry_ID _Assembly_common_name.Assembly_ID 'drosophila SLBP N-terminus' system 5894 1 SLBP abbreviation 5894 1 stop_ loop_ _Assembly_bio_function.Biological_function _Assembly_bio_function.Entry_ID _Assembly_bio_function.Assembly_ID 'Regulation of histone mRNA gene expression' 5894 1 'translation regulator for histone genes' 5894 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_dSLBP_N-term _Entity.Sf_category entity _Entity.Sf_framecode dSLBP_N-term _Entity.Entry_ID 5894 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name 'Stem loop binding protein' _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MGSSHHHHHHSSGLVPRGSH MGSGSLNSSASSISIDVKPT MQSWAQEVRAEFGHSDEASS SLNSSAASCGSLAKKETADG NLESKDGEGREMAFEFLDGV NEVKFERLVKEEK ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states . _Entity.Ambiguous_chem_comp_sites . _Entity.Nstd_monomer . _Entity.Nstd_chirality . _Entity.Nstd_linkage . _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 113 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic . _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 12070 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-28 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no GB AAF56867 . "Stem-loop binding protein [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 2 no GB AAF71752 . "replication-associated histone mRNA stem loop-binding protein [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 3 no GB AAG16723 . "histone mRNA stem-loop binding protein [Drosophila melanogaster]" . . . . . 81.42 276 98.91 98.91 1.04e-54 . . . . 5894 1 4 no GB AAL90413 . "RH47057p [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 5 no GB AAM50696 . "GM06606p [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 6 no REF NP_477480 . "Stem-loop binding protein [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 7 no SP Q9VAN6 . "RecName: Full=Histone RNA hairpin-binding protein; AltName: Full=Histone stem-loop-binding protein [Drosophila melanogaster]" . . . . . 81.42 276 100.00 100.00 6.51e-56 . . . . 5894 1 stop_ loop_ _Entity_common_name.Name _Entity_common_name.Type _Entity_common_name.Entry_ID _Entity_common_name.Entity_ID SLBP abbreviation 5894 1 'Stem loop binding protein' common 5894 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -5 MET . 5894 1 2 -4 GLY . 5894 1 3 -3 SER . 5894 1 4 -2 SER . 5894 1 5 -1 HIS . 5894 1 6 1 HIS . 5894 1 7 2 HIS . 5894 1 8 3 HIS . 5894 1 9 4 HIS . 5894 1 10 5 HIS . 5894 1 11 6 SER . 5894 1 12 7 SER . 5894 1 13 8 GLY . 5894 1 14 9 LEU . 5894 1 15 10 VAL . 5894 1 16 11 PRO . 5894 1 17 12 ARG . 5894 1 18 13 GLY . 5894 1 19 14 SER . 5894 1 20 15 HIS . 5894 1 21 16 MET . 5894 1 22 17 GLY . 5894 1 23 18 SER . 5894 1 24 19 GLY . 5894 1 25 20 SER . 5894 1 26 21 LEU . 5894 1 27 22 ASN . 5894 1 28 23 SER . 5894 1 29 24 SER . 5894 1 30 25 ALA . 5894 1 31 26 SER . 5894 1 32 27 SER . 5894 1 33 28 ILE . 5894 1 34 29 SER . 5894 1 35 30 ILE . 5894 1 36 31 ASP . 5894 1 37 32 VAL . 5894 1 38 33 LYS . 5894 1 39 34 PRO . 5894 1 40 35 THR . 5894 1 41 36 MET . 5894 1 42 37 GLN . 5894 1 43 38 SER . 5894 1 44 39 TRP . 5894 1 45 40 ALA . 5894 1 46 41 GLN . 5894 1 47 42 GLU . 5894 1 48 43 VAL . 5894 1 49 44 ARG . 5894 1 50 45 ALA . 5894 1 51 46 GLU . 5894 1 52 47 PHE . 5894 1 53 48 GLY . 5894 1 54 49 HIS . 5894 1 55 50 SER . 5894 1 56 51 ASP . 5894 1 57 52 GLU . 5894 1 58 53 ALA . 5894 1 59 54 SER . 5894 1 60 55 SER . 5894 1 61 56 SER . 5894 1 62 57 LEU . 5894 1 63 58 ASN . 5894 1 64 59 SER . 5894 1 65 60 SER . 5894 1 66 61 ALA . 5894 1 67 62 ALA . 5894 1 68 63 SER . 5894 1 69 64 CYS . 5894 1 70 65 GLY . 5894 1 71 66 SER . 5894 1 72 67 LEU . 5894 1 73 68 ALA . 5894 1 74 69 LYS . 5894 1 75 70 LYS . 5894 1 76 71 GLU . 5894 1 77 72 THR . 5894 1 78 73 ALA . 5894 1 79 74 ASP . 5894 1 80 75 GLY . 5894 1 81 76 ASN . 5894 1 82 77 LEU . 5894 1 83 78 GLU . 5894 1 84 79 SER . 5894 1 85 80 LYS . 5894 1 86 81 ASP . 5894 1 87 82 GLY . 5894 1 88 83 GLU . 5894 1 89 84 GLY . 5894 1 90 85 ARG . 5894 1 91 86 GLU . 5894 1 92 87 MET . 5894 1 93 88 ALA . 5894 1 94 89 PHE . 5894 1 95 90 GLU . 5894 1 96 91 PHE . 5894 1 97 92 LEU . 5894 1 98 93 ASP . 5894 1 99 94 GLY . 5894 1 100 95 VAL . 5894 1 101 96 ASN . 5894 1 102 97 GLU . 5894 1 103 98 VAL . 5894 1 104 99 LYS . 5894 1 105 100 PHE . 5894 1 106 101 GLU . 5894 1 107 102 ARG . 5894 1 108 103 LEU . 5894 1 109 104 VAL . 5894 1 110 105 LYS . 5894 1 111 106 GLU . 5894 1 112 107 GLU . 5894 1 113 108 LYS . 5894 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 5894 1 . GLY 2 2 5894 1 . SER 3 3 5894 1 . SER 4 4 5894 1 . HIS 5 5 5894 1 . HIS 6 6 5894 1 . HIS 7 7 5894 1 . HIS 8 8 5894 1 . HIS 9 9 5894 1 . HIS 10 10 5894 1 . SER 11 11 5894 1 . SER 12 12 5894 1 . GLY 13 13 5894 1 . LEU 14 14 5894 1 . VAL 15 15 5894 1 . PRO 16 16 5894 1 . ARG 17 17 5894 1 . GLY 18 18 5894 1 . SER 19 19 5894 1 . HIS 20 20 5894 1 . MET 21 21 5894 1 . GLY 22 22 5894 1 . SER 23 23 5894 1 . GLY 24 24 5894 1 . SER 25 25 5894 1 . LEU 26 26 5894 1 . ASN 27 27 5894 1 . SER 28 28 5894 1 . SER 29 29 5894 1 . ALA 30 30 5894 1 . SER 31 31 5894 1 . SER 32 32 5894 1 . ILE 33 33 5894 1 . SER 34 34 5894 1 . ILE 35 35 5894 1 . ASP 36 36 5894 1 . VAL 37 37 5894 1 . LYS 38 38 5894 1 . PRO 39 39 5894 1 . THR 40 40 5894 1 . MET 41 41 5894 1 . GLN 42 42 5894 1 . SER 43 43 5894 1 . TRP 44 44 5894 1 . ALA 45 45 5894 1 . GLN 46 46 5894 1 . GLU 47 47 5894 1 . VAL 48 48 5894 1 . ARG 49 49 5894 1 . ALA 50 50 5894 1 . GLU 51 51 5894 1 . PHE 52 52 5894 1 . GLY 53 53 5894 1 . HIS 54 54 5894 1 . SER 55 55 5894 1 . ASP 56 56 5894 1 . GLU 57 57 5894 1 . ALA 58 58 5894 1 . SER 59 59 5894 1 . SER 60 60 5894 1 . SER 61 61 5894 1 . LEU 62 62 5894 1 . ASN 63 63 5894 1 . SER 64 64 5894 1 . SER 65 65 5894 1 . ALA 66 66 5894 1 . ALA 67 67 5894 1 . SER 68 68 5894 1 . CYS 69 69 5894 1 . GLY 70 70 5894 1 . SER 71 71 5894 1 . LEU 72 72 5894 1 . ALA 73 73 5894 1 . LYS 74 74 5894 1 . LYS 75 75 5894 1 . GLU 76 76 5894 1 . THR 77 77 5894 1 . ALA 78 78 5894 1 . ASP 79 79 5894 1 . GLY 80 80 5894 1 . ASN 81 81 5894 1 . LEU 82 82 5894 1 . GLU 83 83 5894 1 . SER 84 84 5894 1 . LYS 85 85 5894 1 . ASP 86 86 5894 1 . GLY 87 87 5894 1 . GLU 88 88 5894 1 . GLY 89 89 5894 1 . ARG 90 90 5894 1 . GLU 91 91 5894 1 . MET 92 92 5894 1 . ALA 93 93 5894 1 . PHE 94 94 5894 1 . GLU 95 95 5894 1 . PHE 96 96 5894 1 . LEU 97 97 5894 1 . ASP 98 98 5894 1 . GLY 99 99 5894 1 . VAL 100 100 5894 1 . ASN 101 101 5894 1 . GLU 102 102 5894 1 . VAL 103 103 5894 1 . LYS 104 104 5894 1 . PHE 105 105 5894 1 . GLU 106 106 5894 1 . ARG 107 107 5894 1 . LEU 108 108 5894 1 . VAL 109 109 5894 1 . LYS 110 110 5894 1 . GLU 111 111 5894 1 . GLU 112 112 5894 1 . LYS 113 113 5894 1 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 5894 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $dSLBP_N-term . 7227 organism . 'Drosophila melanogaster' 'Fruit fly' . . Eukaryota Metazoa Drosophila melanogaster . . . . . . . . . . . . . . . . . . . . . 5894 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 5894 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $dSLBP_N-term . 'recombinant technology' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5894 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_Sample_1 _Sample.Sf_category sample _Sample.Sf_framecode Sample_1 _Sample.Entry_ID 5894 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system . _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Stem loop binding protein' '[U-15N; U-13C]' . . 1 $dSLBP_N-term . . 2 . . mM . . . . 5894 1 stop_ save_ ####################### # Sample conditions # ####################### save_condition_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode condition_1 _Sample_condition_list.Entry_ID 5894 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 0.050 0.01 M 5894 1 pH 6.0 0.1 n/a 5894 1 temperature 298 2 K 5894 1 stop_ save_ ############################ # Computer software used # ############################ save_FELIX _Software.Sf_category software _Software.Sf_framecode FELIX _Software.Entry_ID 5894 _Software.ID 1 _Software.Name FELIX _Software.Version . _Software.Details . loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 5894 1 'data processing' 5894 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode NMR_spectrometer _NMR_spectrometer.Entry_ID 5894 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model INOVA _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode spectrometer_list _NMR_spectrometer_list.Entry_ID 5894 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 NMR_spectrometer Varian INOVA . 600 . . . 5894 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 5894 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '(15N,1H) HSQC' . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 2 HNCA . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 3 HN(CO)CA . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 4 HNCO . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 5 HNCACB . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 6 'CC(CO)NH TOCSY' . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 7 HCCH-TOCSY . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 8 HNHA . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 9 '1H-15N NOESY' . . . . . . . . . . . 1 $Sample_1 . . . 1 $condition_1 . . . 1 $NMR_spectrometer . . . . . . . . . . . . . . . . 5894 1 stop_ save_ save_NMR_spec_expt__0_1 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_1 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 1 _NMR_spec_expt.Name '(15N,1H) HSQC' _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_2 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_2 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 2 _NMR_spec_expt.Name HNCA _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_3 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_3 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 3 _NMR_spec_expt.Name HN(CO)CA _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_4 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_4 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 4 _NMR_spec_expt.Name HNCO _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_5 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_5 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 5 _NMR_spec_expt.Name HNCACB _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_6 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_6 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 6 _NMR_spec_expt.Name 'CC(CO)NH TOCSY' _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_7 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_7 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 7 _NMR_spec_expt.Name HCCH-TOCSY _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_8 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_8 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 8 _NMR_spec_expt.Name HNHA _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ save_NMR_spec_expt__0_9 _NMR_spec_expt.Sf_category NMR_spectrometer_expt _NMR_spec_expt.Sf_framecode NMR_spec_expt__0_9 _NMR_spec_expt.Entry_ID 5894 _NMR_spec_expt.ID 9 _NMR_spec_expt.Name '1H-15N NOESY' _NMR_spec_expt.Type . _NMR_spec_expt.Sample_volume . _NMR_spec_expt.Sample_volume_units . _NMR_spec_expt.NMR_tube_type . _NMR_spec_expt.Sample_spinning_rate . _NMR_spec_expt.Sample_angle . _NMR_spec_expt.NMR_spectrometer_ID 1 _NMR_spec_expt.NMR_spectrometer_label $NMR_spectrometer _NMR_spec_expt.NMR_spectrometer_probe_ID . _NMR_spec_expt.NMR_spectrometer_probe_label . _NMR_spec_expt.Carrier_freq_switch_time . _NMR_spec_expt.Software_ID 1 _NMR_spec_expt.Software_label $FELIX _NMR_spec_expt.Method_ID . _NMR_spec_expt.Method_label . _NMR_spec_expt.Pulse_seq_accession_BMRB_code . _NMR_spec_expt.Details '5 mm z-gradient Triax probe' save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference _Chem_shift_reference.Entry_ID 5894 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio_cit_ID _Chem_shift_ref.Indirect_shift_ratio_cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_cit_ID _Chem_shift_ref.Correction_val_cit_label _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID C 13 DSS 'methyl protons' . . . . ppm 0.0 . indirect 0.251449530 . . . . . . . . . 5894 1 H 1 DSS 'methyl protons' . . . . ppm 0.0 internal direct 1.0 . . . . . . . . . 5894 1 N 15 DSS 'methyl protons' . . . . ppm 0.0 . indirect 0.101329118 . . . . . . . . . 5894 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode shift_set_1 _Assigned_chem_shift_list.Entry_ID 5894 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $condition_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID . . 1 $Sample_1 . 5894 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 22 22 GLY H H 1 8.41 0.02 . 1 . . . . . . . . 5894 1 2 . 1 1 22 22 GLY N N 15 109.08 0.05 . 1 . . . . . . . . 5894 1 3 . 1 1 22 22 GLY CA C 13 46.81 0.05 . 1 . . . . . . . . 5894 1 4 . 1 1 23 23 SER H H 1 8.15 0.02 . 1 . . . . . . . . 5894 1 5 . 1 1 23 23 SER N N 15 114.28 0.05 . 1 . . . . . . . . 5894 1 6 . 1 1 23 23 SER CA C 13 60 0.05 . 1 . . . . . . . . 5894 1 7 . 1 1 23 23 SER C C 13 174.11 0.05 . 1 . . . . . . . . 5894 1 8 . 1 1 23 23 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 9 . 1 1 24 24 GLY H H 1 8.48 0.02 . 1 . . . . . . . . 5894 1 10 . 1 1 24 24 GLY N N 15 109.89 0.05 . 1 . . . . . . . . 5894 1 11 . 1 1 24 24 GLY CA C 13 46.82 0.05 . 1 . . . . . . . . 5894 1 12 . 1 1 25 25 SER H H 1 8.29 0.02 . 1 . . . . . . . . 5894 1 13 . 1 1 25 25 SER N N 15 114.53 0.05 . 1 . . . . . . . . 5894 1 14 . 1 1 25 25 SER CA C 13 59.49 0.05 . 1 . . . . . . . . 5894 1 15 . 1 1 25 25 SER HA H 1 3.94 0.02 . 1 . . . . . . . . 5894 1 16 . 1 1 25 25 SER C C 13 172.25 0.05 . 1 . . . . . . . . 5894 1 17 . 1 1 25 25 SER CB C 13 65.33 0.05 . 1 . . . . . . . . 5894 1 18 . 1 1 26 26 LEU H H 1 8.27 0.02 . 1 . . . . . . . . 5894 1 19 . 1 1 26 26 LEU N N 15 122.48 0.05 . 1 . . . . . . . . 5894 1 20 . 1 1 26 26 LEU CA C 13 55.69 0.05 . 1 . . . . . . . . 5894 1 21 . 1 1 26 26 LEU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 22 . 1 1 26 26 LEU C C 13 172.9 0.05 . 1 . . . . . . . . 5894 1 23 . 1 1 26 26 LEU CB C 13 43.51 0.05 . 1 . . . . . . . . 5894 1 24 . 1 1 26 26 LEU CG C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 25 . 1 1 26 26 LEU CD1 C 13 26.25 0.05 . 1 . . . . . . . . 5894 1 26 . 1 1 26 26 LEU CD2 C 13 24.73 0.05 . 1 . . . . . . . . 5894 1 27 . 1 1 27 27 ASN H H 1 8.34 0.02 . 1 . . . . . . . . 5894 1 28 . 1 1 27 27 ASN N N 15 119.97 0.05 . 1 . . . . . . . . 5894 1 29 . 1 1 27 27 ASN CA C 13 57.83 0.05 . 1 . . . . . . . . 5894 1 30 . 1 1 27 27 ASN HA H 1 4.16 0.02 . 1 . . . . . . . . 5894 1 31 . 1 1 27 27 ASN C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 32 . 1 1 27 27 ASN CB C 13 31.63 0.05 . 1 . . . . . . . . 5894 1 33 . 1 1 32 32 SER H H 1 8.18 0.02 . 1 . . . . . . . . 5894 1 34 . 1 1 32 32 SER N N 15 119.69 0.05 . 1 . . . . . . . . 5894 1 35 . 1 1 32 32 SER CA C 13 59.24 0.05 . 1 . . . . . . . . 5894 1 36 . 1 1 32 32 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 37 . 1 1 33 33 ILE H H 1 8.12 0.02 . 1 . . . . . . . . 5894 1 38 . 1 1 33 33 ILE N N 15 121.02 0.05 . 1 . . . . . . . . 5894 1 39 . 1 1 33 33 ILE CA C 13 62.28 0.05 . 1 . . . . . . . . 5894 1 40 . 1 1 33 33 ILE C C 13 172.03 0.05 . 1 . . . . . . . . 5894 1 41 . 1 1 33 33 ILE CB C 13 40.2 0.05 . 1 . . . . . . . . 5894 1 42 . 1 1 33 33 ILE CD1 C 13 18.88 0.05 . 1 . . . . . . . . 5894 1 43 . 1 1 33 33 ILE CG1 C 13 28.53 0.05 . 1 . . . . . . . . 5894 1 44 . 1 1 33 33 ILE CG2 C 13 24.2 0.05 . 1 . . . . . . . . 5894 1 45 . 1 1 34 34 SER H H 1 8.28 0.02 . 1 . . . . . . . . 5894 1 46 . 1 1 34 34 SER N N 15 118.61 0.05 . 1 . . . . . . . . 5894 1 47 . 1 1 34 34 SER CA C 13 59.49 0.05 . 1 . . . . . . . . 5894 1 48 . 1 1 34 34 SER HA H 1 4.46 0.02 . 1 . . . . . . . . 5894 1 49 . 1 1 34 34 SER C C 13 173.12 0.05 . 1 . . . . . . . . 5894 1 50 . 1 1 34 34 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 51 . 1 1 35 35 ILE H H 1 8.01 0.02 . 1 . . . . . . . . 5894 1 52 . 1 1 35 35 ILE N N 15 120.49 0.05 . 1 . . . . . . . . 5894 1 53 . 1 1 35 35 ILE CA C 13 62.54 0.05 . 1 . . . . . . . . 5894 1 54 . 1 1 35 35 ILE C C 13 175.2 0.05 . 1 . . . . . . . . 5894 1 55 . 1 1 35 35 ILE CB C 13 40.46 0.05 . 1 . . . . . . . . 5894 1 56 . 1 1 35 35 ILE CD1 C 13 18.88 0.05 . 1 . . . . . . . . 5894 1 57 . 1 1 35 35 ILE CG1 C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 58 . 1 1 35 35 ILE CG2 C 13 24.2 0.05 . 1 . . . . . . . . 5894 1 59 . 1 1 36 36 ASP H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 60 . 1 1 36 36 ASP N N 15 122.71 0.05 . 1 . . . . . . . . 5894 1 61 . 1 1 36 36 ASP CA C 13 55.69 0.05 . 1 . . . . . . . . 5894 1 62 . 1 1 36 36 ASP HA H 1 4.58 0.02 . 1 . . . . . . . . 5894 1 63 . 1 1 36 36 ASP C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 64 . 1 1 36 36 ASP CB C 13 42.49 0.05 . 1 . . . . . . . . 5894 1 65 . 1 1 37 37 VAL H H 1 7.96 0.02 . 1 . . . . . . . . 5894 1 66 . 1 1 37 37 VAL N N 15 119.36 0.05 . 1 . . . . . . . . 5894 1 67 . 1 1 37 37 VAL CA C 13 64.32 0.05 . 1 . . . . . . . . 5894 1 68 . 1 1 37 37 VAL HA H 1 4.07 0.05 . 1 . . . . . . . . 5894 1 69 . 1 1 37 37 VAL C C 13 175.2 0.05 . 1 . . . . . . . . 5894 1 70 . 1 1 37 37 VAL CB C 13 33.35 0.05 . 1 . . . . . . . . 5894 1 71 . 1 1 37 37 VAL CG1 C 13 28.78 0.05 . 1 . . . . . . . . 5894 1 72 . 1 1 38 38 LYS H H 1 8.42 0.02 . 1 . . . . . . . . 5894 1 73 . 1 1 38 38 LYS N N 15 120.66 0.05 . 1 . . . . . . . . 5894 1 74 . 1 1 38 38 LYS CA C 13 57.65 0.05 . 1 . . . . . . . . 5894 1 75 . 1 1 38 38 LYS HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 76 . 1 1 38 38 LYS C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 77 . 1 1 38 38 LYS CB C 13 32.15 0.05 . 1 . . . . . . . . 5894 1 78 . 1 1 39 39 PRO CA C 13 64.57 0.05 . 1 . . . . . . . . 5894 1 79 . 1 1 39 39 PRO CD C 13 46.55 0.05 . 1 . . . . . . . . 5894 1 80 . 1 1 39 39 PRO CG C 13 28.53 0.05 . 1 . . . . . . . . 5894 1 81 . 1 1 40 40 THR H H 1 8.16 0.02 . 1 . . . . . . . . 5894 1 82 . 1 1 40 40 THR N N 15 112.9 0.05 . 1 . . . . . . . . 5894 1 83 . 1 1 40 40 THR CA C 13 59.75 0.05 . 1 . . . . . . . . 5894 1 84 . 1 1 40 40 THR C C 13 173.02 0.05 . 1 . . . . . . . . 5894 1 85 . 1 1 40 40 THR CB C 13 64.82 0.05 . 1 . . . . . . . . 5894 1 86 . 1 1 41 41 MET H H 1 8.19 0.02 . 1 . . . . . . . . 5894 1 87 . 1 1 41 41 MET N N 15 124.46 0.05 . 1 . . . . . . . . 5894 1 88 . 1 1 41 41 MET CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 89 . 1 1 41 41 MET C C 13 174.66 0.05 . 1 . . . . . . . . 5894 1 90 . 1 1 41 41 MET CB C 13 32.34 0.05 . 1 . . . . . . . . 5894 1 91 . 1 1 41 41 MET CG C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 92 . 1 1 42 42 GLN H H 1 8.56 0.02 . 1 . . . . . . . . 5894 1 93 . 1 1 42 42 GLN N N 15 120.57 0.05 . 1 . . . . . . . . 5894 1 94 . 1 1 42 42 GLN CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 95 . 1 1 42 42 GLN HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 96 . 1 1 42 42 GLN C C 13 173.12 0.05 . 1 . . . . . . . . 5894 1 97 . 1 1 42 42 GLN CB C 13 30.56 0.05 . 1 . . . . . . . . 5894 1 98 . 1 1 42 42 GLN CG C 13 34.88 0.05 . 1 . . . . . . . . 5894 1 99 . 1 1 43 43 SER H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 100 . 1 1 43 43 SER N N 15 115.57 0.05 . 1 . . . . . . . . 5894 1 101 . 1 1 43 43 SER CA C 13 59.75 0.05 . 1 . . . . . . . . 5894 1 102 . 1 1 43 43 SER C C 13 174.98 0.05 . 1 . . . . . . . . 5894 1 103 . 1 1 43 43 SER CB C 13 64.82 0.05 . 1 . . . . . . . . 5894 1 104 . 1 1 44 44 TRP H H 1 7.98 0.02 . 1 . . . . . . . . 5894 1 105 . 1 1 44 44 TRP N N 15 121.35 0.05 . 1 . . . . . . . . 5894 1 106 . 1 1 44 44 TRP CA C 13 58.48 0.05 . 1 . . . . . . . . 5894 1 107 . 1 1 44 44 TRP HA H 1 4.67 0.02 . 1 . . . . . . . . 5894 1 108 . 1 1 44 44 TRP C C 13 173.78 0.05 . 1 . . . . . . . . 5894 1 109 . 1 1 44 44 TRP CB C 13 30.56 0.05 . 1 . . . . . . . . 5894 1 110 . 1 1 45 45 ALA H H 1 7.94 0.02 . 1 . . . . . . . . 5894 1 111 . 1 1 45 45 ALA N N 15 123.51 0.05 . 1 . . . . . . . . 5894 1 112 . 1 1 45 45 ALA CA C 13 53.91 0.05 . 1 . . . . . . . . 5894 1 113 . 1 1 45 45 ALA HA H 1 4.15 0.02 . 1 . . . . . . . . 5894 1 114 . 1 1 45 45 ALA C C 13 175.2 0.05 . 1 . . . . . . . . 5894 1 115 . 1 1 45 45 ALA CB C 13 20.66 0.05 . 1 . . . . . . . . 5894 1 116 . 1 1 46 46 GLN H H 1 8.33 0.02 . 1 . . . . . . . . 5894 1 117 . 1 1 46 46 GLN N N 15 118.65 0.05 . 1 . . . . . . . . 5894 1 118 . 1 1 46 46 GLN CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 119 . 1 1 46 46 GLN HA H 1 4.16 0.02 . 1 . . . . . . . . 5894 1 120 . 1 1 46 46 GLN C C 13 173.12 0.05 . 1 . . . . . . . . 5894 1 121 . 1 1 46 46 GLN CB C 13 31.57 0.05 . 1 . . . . . . . . 5894 1 122 . 1 1 46 46 GLN CG C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 123 . 1 1 47 47 GLU H H 1 8.14 0.02 . 1 . . . . . . . . 5894 1 124 . 1 1 47 47 GLU N N 15 120.59 0.05 . 1 . . . . . . . . 5894 1 125 . 1 1 47 47 GLU CA C 13 57.13 0.05 . 1 . . . . . . . . 5894 1 126 . 1 1 47 47 GLU HA H 1 4.02 0.02 . 1 . . . . . . . . 5894 1 127 . 1 1 47 47 GLU C C 13 171.81 0.05 . 1 . . . . . . . . 5894 1 128 . 1 1 47 47 GLU CB C 13 33.61 0.05 . 1 . . . . . . . . 5894 1 129 . 1 1 48 48 VAL H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 130 . 1 1 48 48 VAL N N 15 123.88 0.05 . 1 . . . . . . . . 5894 1 131 . 1 1 48 48 VAL CA C 13 63.3 0.05 . 1 . . . . . . . . 5894 1 132 . 1 1 48 48 VAL HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 133 . 1 1 48 48 VAL C C 13 174.76 0.05 . 1 . . . . . . . . 5894 1 134 . 1 1 48 48 VAL CB C 13 34.12 0.05 . 1 . . . . . . . . 5894 1 135 . 1 1 48 48 VAL CG1 C 13 22.44 0.05 . 1 . . . . . . . . 5894 1 136 . 1 1 49 49 ARG H H 1 8.32 0.02 . 1 . . . . . . . . 5894 1 137 . 1 1 49 49 ARG N N 15 125.33 0.05 . 1 . . . . . . . . 5894 1 138 . 1 1 49 49 ARG CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 139 . 1 1 49 49 ARG HA H 1 4.55 0.02 . 1 . . . . . . . . 5894 1 140 . 1 1 49 49 ARG C C 13 172.79 0.05 . 1 . . . . . . . . 5894 1 141 . 1 1 49 49 ARG CB C 13 32.08 0.05 . 1 . . . . . . . . 5894 1 142 . 1 1 49 49 ARG CG C 13 28.53 0.05 . 1 . . . . . . . . 5894 1 143 . 1 1 50 50 ALA H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 144 . 1 1 50 50 ALA N N 15 124.52 0.05 . 1 . . . . . . . . 5894 1 145 . 1 1 50 50 ALA CA C 13 54.17 0.05 . 1 . . . . . . . . 5894 1 146 . 1 1 50 50 ALA HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 147 . 1 1 50 50 ALA C C 13 174.54 0.05 . 1 . . . . . . . . 5894 1 148 . 1 1 50 50 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 149 . 1 1 51 51 GLU H H 1 8.13 0.02 . 1 . . . . . . . . 5894 1 150 . 1 1 51 51 GLU N N 15 121.23 0.05 . 1 . . . . . . . . 5894 1 151 . 1 1 51 51 GLU CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 152 . 1 1 51 51 GLU HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 153 . 1 1 51 51 GLU C C 13 174.87 0.05 . 1 . . . . . . . . 5894 1 154 . 1 1 51 51 GLU CB C 13 34.37 0.05 . 1 . . . . . . . . 5894 1 155 . 1 1 52 52 PHE H H 1 8.176 0.02 . 1 . . . . . . . . 5894 1 156 . 1 1 52 52 PHE N N 15 120.29 0.05 . 1 . . . . . . . . 5894 1 157 . 1 1 52 52 PHE CA C 13 58.88 0.05 . 1 . . . . . . . . 5894 1 158 . 1 1 52 52 PHE HA H 1 4.54 0.02 . 1 . . . . . . . . 5894 1 159 . 1 1 52 52 PHE C C 13 175.2 0.05 . 1 . . . . . . . . 5894 1 160 . 1 1 52 52 PHE CB C 13 40.89 0.05 . 1 . . . . . . . . 5894 1 161 . 1 1 53 53 GLY H H 1 8.41 0.02 . 1 . . . . . . . . 5894 1 162 . 1 1 53 53 GLY N N 15 127.94 0.05 . 1 . . . . . . . . 5894 1 163 . 1 1 53 53 GLY CA C 13 46.55 0.05 . 1 . . . . . . . . 5894 1 164 . 1 1 54 54 HIS H H 1 8.28 0.02 . 1 . . . . . . . . 5894 1 165 . 1 1 54 54 HIS N N 15 117.38 0.05 . 1 . . . . . . . . 5894 1 166 . 1 1 54 54 HIS CA C 13 54.42 0.05 . 1 . . . . . . . . 5894 1 167 . 1 1 54 54 HIS HA H 1 4.72 0.02 . 1 . . . . . . . . 5894 1 168 . 1 1 54 54 HIS C C 13 172.79 0.05 . 1 . . . . . . . . 5894 1 169 . 1 1 54 54 HIS CB C 13 40.21 0.05 . 1 . . . . . . . . 5894 1 170 . 1 1 55 55 SER H H 1 8.2 0.02 . 1 . . . . . . . . 5894 1 171 . 1 1 55 55 SER N N 15 115.01 0.05 . 1 . . . . . . . . 5894 1 172 . 1 1 55 55 SER CA C 13 59.75 0.05 . 1 . . . . . . . . 5894 1 173 . 1 1 55 55 SER C C 13 173.78 0.05 . 1 . . . . . . . . 5894 1 174 . 1 1 55 55 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 175 . 1 1 56 56 ASP H H 1 8.2 0.02 . 1 . . . . . . . . 5894 1 176 . 1 1 56 56 ASP N N 15 122.7 0.05 . 1 . . . . . . . . 5894 1 177 . 1 1 56 56 ASP CA C 13 57.46 0.05 . 1 . . . . . . . . 5894 1 178 . 1 1 56 56 ASP HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 179 . 1 1 56 56 ASP C C 13 174 0.05 . 1 . . . . . . . . 5894 1 180 . 1 1 56 56 ASP CB C 13 43.25 0.05 . 1 . . . . . . . . 5894 1 181 . 1 1 57 57 GLU H H 1 8.42 0.02 . 1 . . . . . . . . 5894 1 182 . 1 1 57 57 GLU N N 15 121.85 0.05 . 1 . . . . . . . . 5894 1 183 . 1 1 57 57 GLU CA C 13 58.23 0.05 . 1 . . . . . . . . 5894 1 184 . 1 1 57 57 GLU HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 185 . 1 1 57 57 GLU C C 13 174.88 0.05 . 1 . . . . . . . . 5894 1 186 . 1 1 57 57 GLU CB C 13 31.33 0.05 . 1 . . . . . . . . 5894 1 187 . 1 1 57 57 GLU CG C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 188 . 1 1 58 58 ALA H H 1 8.29 0.02 . 1 . . . . . . . . 5894 1 189 . 1 1 58 58 ALA N N 15 123.19 0.05 . 1 . . . . . . . . 5894 1 190 . 1 1 58 58 ALA CA C 13 54.16 0.05 . 1 . . . . . . . . 5894 1 191 . 1 1 58 58 ALA HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 192 . 1 1 58 58 ALA C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 193 . 1 1 58 58 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 194 . 1 1 59 59 SER H H 1 8.16 0.02 . 1 . . . . . . . . 5894 1 195 . 1 1 59 59 SER N N 15 113.24 0.05 . 1 . . . . . . . . 5894 1 196 . 1 1 59 59 SER CA C 13 59.75 0.05 . 1 . . . . . . . . 5894 1 197 . 1 1 59 59 SER HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 198 . 1 1 59 59 SER C C 13 173.01 0.05 . 1 . . . . . . . . 5894 1 199 . 1 1 59 59 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 200 . 1 1 60 60 SER H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 201 . 1 1 60 60 SER N N 15 116.35 0.05 . 1 . . . . . . . . 5894 1 202 . 1 1 60 60 SER CA C 13 60 0.05 . 1 . . . . . . . . 5894 1 203 . 1 1 60 60 SER HA H 1 4.37 0.02 . 1 . . . . . . . . 5894 1 204 . 1 1 60 60 SER C C 13 175.09 0.05 . 1 . . . . . . . . 5894 1 205 . 1 1 60 60 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 206 . 1 1 61 61 SER H H 1 8.33 0.02 . 1 . . . . . . . . 5894 1 207 . 1 1 61 61 SER N N 15 116.58 0.05 . 1 . . . . . . . . 5894 1 208 . 1 1 61 61 SER CA C 13 59.49 0.05 . 1 . . . . . . . . 5894 1 209 . 1 1 61 61 SER C C 13 175.53 0.05 . 1 . . . . . . . . 5894 1 210 . 1 1 61 61 SER CB C 13 65.33 0.05 . 1 . . . . . . . . 5894 1 211 . 1 1 62 62 LEU H H 1 8.52 0.02 . 1 . . . . . . . . 5894 1 212 . 1 1 62 62 LEU N N 15 121.48 0.05 . 1 . . . . . . . . 5894 1 213 . 1 1 62 62 LEU CA C 13 55.74 0.05 . 1 . . . . . . . . 5894 1 214 . 1 1 62 62 LEU HA H 1 4.59 0.02 . 1 . . . . . . . . 5894 1 215 . 1 1 62 62 LEU CB C 13 42.45 0.05 . 1 . . . . . . . . 5894 1 216 . 1 1 65 65 SER CA C 13 59.75 0.05 . 1 . . . . . . . . 5894 1 217 . 1 1 65 65 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 218 . 1 1 66 66 ALA H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 219 . 1 1 66 66 ALA N N 15 124.43 0.05 . 1 . . . . . . . . 5894 1 220 . 1 1 66 66 ALA CA C 13 53.66 0.05 . 1 . . . . . . . . 5894 1 221 . 1 1 66 66 ALA HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 222 . 1 1 66 66 ALA C C 13 174.66 0.05 . 1 . . . . . . . . 5894 1 223 . 1 1 66 66 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 224 . 1 1 67 67 ALA H H 1 8.16 0.02 . 1 . . . . . . . . 5894 1 225 . 1 1 67 67 ALA N N 15 119.67 0.05 . 1 . . . . . . . . 5894 1 226 . 1 1 67 67 ALA CA C 13 57.31 0.05 . 1 . . . . . . . . 5894 1 227 . 1 1 67 67 ALA C C 13 172.58 0.05 . 1 . . . . . . . . 5894 1 228 . 1 1 67 67 ALA CB C 13 20.42 0.05 . 1 . . . . . . . . 5894 1 229 . 1 1 69 69 CYS CA C 13 59.05 0.05 . 1 . . . . . . . . 5894 1 230 . 1 1 69 69 CYS CB C 13 40.88 0.05 . 1 . . . . . . . . 5894 1 231 . 1 1 70 70 GLY H H 1 8.35 0.02 . 1 . . . . . . . . 5894 1 232 . 1 1 70 70 GLY N N 15 108.88 0.05 . 1 . . . . . . . . 5894 1 233 . 1 1 70 70 GLY CA C 13 46.47 0.05 . 1 . . . . . . . . 5894 1 234 . 1 1 71 71 SER H H 1 8.17 0.02 . 1 . . . . . . . . 5894 1 235 . 1 1 71 71 SER N N 15 114.42 0.05 . 1 . . . . . . . . 5894 1 236 . 1 1 71 71 SER CA C 13 60 0.05 . 1 . . . . . . . . 5894 1 237 . 1 1 71 71 SER C C 13 172.91 0.05 . 1 . . . . . . . . 5894 1 238 . 1 1 71 71 SER CB C 13 64.82 0.05 . 1 . . . . . . . . 5894 1 239 . 1 1 72 72 LEU H H 1 8.33 0.02 . 1 . . . . . . . . 5894 1 240 . 1 1 72 72 LEU N N 15 122.17 0.05 . 1 . . . . . . . . 5894 1 241 . 1 1 72 72 LEU CA C 13 56.45 0.05 . 1 . . . . . . . . 5894 1 242 . 1 1 72 72 LEU HA H 1 4.02 0.02 . 1 . . . . . . . . 5894 1 243 . 1 1 72 72 LEU C C 13 174 0.05 . 1 . . . . . . . . 5894 1 244 . 1 1 72 72 LEU CB C 13 43.51 0.05 . 1 . . . . . . . . 5894 1 245 . 1 1 72 72 LEU CG C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 246 . 1 1 72 72 LEU CD1 C 13 26.25 0.05 . 1 . . . . . . . . 5894 1 247 . 1 1 72 72 LEU CD2 C 13 24.72 0.05 . 1 . . . . . . . . 5894 1 248 . 1 1 73 73 ALA H H 1 8.09 0.02 . 1 . . . . . . . . 5894 1 249 . 1 1 73 73 ALA N N 15 123.23 0.05 . 1 . . . . . . . . 5894 1 250 . 1 1 73 73 ALA CA C 13 53.91 0.05 . 1 . . . . . . . . 5894 1 251 . 1 1 73 73 ALA HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 252 . 1 1 73 73 ALA C C 13 172.25 0.05 . 1 . . . . . . . . 5894 1 253 . 1 1 73 73 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 254 . 1 1 74 74 LYS H H 1 8.02 0.02 . 1 . . . . . . . . 5894 1 255 . 1 1 74 74 LYS N N 15 117.64 0.05 . 1 . . . . . . . . 5894 1 256 . 1 1 74 74 LYS CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 257 . 1 1 74 74 LYS HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 258 . 1 1 74 74 LYS C C 13 172.47 0.05 . 1 . . . . . . . . 5894 1 259 . 1 1 74 74 LYS CB C 13 31.07 0.05 . 1 . . . . . . . . 5894 1 260 . 1 1 74 74 LYS CG C 13 27.67 0.05 . 1 . . . . . . . . 5894 1 261 . 1 1 75 75 LYS H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 262 . 1 1 75 75 LYS N N 15 120.26 0.05 . 1 . . . . . . . . 5894 1 263 . 1 1 75 75 LYS CA C 13 57.72 0.05 . 1 . . . . . . . . 5894 1 264 . 1 1 75 75 LYS HA H 1 4.41 0.02 . 1 . . . . . . . . 5894 1 265 . 1 1 75 75 LYS C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 266 . 1 1 75 75 LYS CB C 13 34.62 0.05 . 1 . . . . . . . . 5894 1 267 . 1 1 75 75 LYS CG C 13 25.99 0.05 . 1 . . . . . . . . 5894 1 268 . 1 1 75 75 LYS CE C 13 43.51 0.05 . 1 . . . . . . . . 5894 1 269 . 1 1 76 76 GLU H H 1 8.55 0.02 . 1 . . . . . . . . 5894 1 270 . 1 1 76 76 GLU N N 15 121.39 0.05 . 1 . . . . . . . . 5894 1 271 . 1 1 76 76 GLU CA C 13 57.83 0.05 . 1 . . . . . . . . 5894 1 272 . 1 1 76 76 GLU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 273 . 1 1 76 76 GLU C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 274 . 1 1 76 76 GLU CB C 13 31.63 0.05 . 1 . . . . . . . . 5894 1 275 . 1 1 77 77 THR H H 1 8.13 0.02 . 1 . . . . . . . . 5894 1 276 . 1 1 77 77 THR N N 15 113.63 0.05 . 1 . . . . . . . . 5894 1 277 . 1 1 77 77 THR CA C 13 62.23 0.05 . 1 . . . . . . . . 5894 1 278 . 1 1 77 77 THR HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 279 . 1 1 77 77 THR C C 13 175.31 0.05 . 1 . . . . . . . . 5894 1 280 . 1 1 77 77 THR CB C 13 63.05 0.05 . 1 . . . . . . . . 5894 1 281 . 1 1 78 78 ALA H H 1 8.38 0.02 . 1 . . . . . . . . 5894 1 282 . 1 1 78 78 ALA N N 15 125.15 0.05 . 1 . . . . . . . . 5894 1 283 . 1 1 78 78 ALA CA C 13 53.91 0.05 . 1 . . . . . . . . 5894 1 284 . 1 1 78 78 ALA HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 285 . 1 1 78 78 ALA C C 13 173.12 0.05 . 1 . . . . . . . . 5894 1 286 . 1 1 78 78 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 287 . 1 1 79 79 ASP H H 1 8.05 0.02 . 1 . . . . . . . . 5894 1 288 . 1 1 79 79 ASP N N 15 117.89 0.05 . 1 . . . . . . . . 5894 1 289 . 1 1 79 79 ASP CA C 13 58.99 0.05 . 1 . . . . . . . . 5894 1 290 . 1 1 79 79 ASP HA H 1 4.5 0.02 . 1 . . . . . . . . 5894 1 291 . 1 1 79 79 ASP C C 13 174 0.05 . 1 . . . . . . . . 5894 1 292 . 1 1 79 79 ASP CB C 13 40.72 0.05 . 1 . . . . . . . . 5894 1 293 . 1 1 80 80 GLY H H 1 8.26 0.02 . 1 . . . . . . . . 5894 1 294 . 1 1 80 80 GLY N N 15 108.97 0.05 . 1 . . . . . . . . 5894 1 295 . 1 1 80 80 GLY CA C 13 42.49 0.05 . 1 . . . . . . . . 5894 1 296 . 1 1 80 80 GLY HA2 H 1 3.85 0.02 . 1 . . . . . . . . 5894 1 297 . 1 1 81 81 ASN H H 1 8 0.02 . 1 . . . . . . . . 5894 1 298 . 1 1 81 81 ASN N N 15 120.26 0.05 . 1 . . . . . . . . 5894 1 299 . 1 1 81 81 ASN CA C 13 58.99 0.05 . 1 . . . . . . . . 5894 1 300 . 1 1 81 81 ASN HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 301 . 1 1 81 81 ASN C C 13 175.31 0.05 . 1 . . . . . . . . 5894 1 302 . 1 1 81 81 ASN CB C 13 40.97 0.05 . 1 . . . . . . . . 5894 1 303 . 1 1 82 82 LEU H H 1 8.2 0.02 . 1 . . . . . . . . 5894 1 304 . 1 1 82 82 LEU N N 15 120.56 0.05 . 1 . . . . . . . . 5894 1 305 . 1 1 82 82 LEU CA C 13 57.46 0.05 . 1 . . . . . . . . 5894 1 306 . 1 1 82 82 LEU HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 307 . 1 1 82 82 LEU C C 13 174.76 0.05 . 1 . . . . . . . . 5894 1 308 . 1 1 82 82 LEU CB C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 309 . 1 1 82 82 LEU CG C 13 34.37 0.05 . 1 . . . . . . . . 5894 1 310 . 1 1 82 82 LEU CD1 C 13 31.83 0.05 . 1 . . . . . . . . 5894 1 311 . 1 1 82 82 LEU CD2 C 13 25.74 0.05 . 1 . . . . . . . . 5894 1 312 . 1 1 83 83 GLU H H 1 8.42 0.02 . 1 . . . . . . . . 5894 1 313 . 1 1 83 83 GLU N N 15 122.17 0.05 . 1 . . . . . . . . 5894 1 314 . 1 1 83 83 GLU CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 315 . 1 1 83 83 GLU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 316 . 1 1 83 83 GLU CB C 13 37.67 0.05 . 1 . . . . . . . . 5894 1 317 . 1 1 83 83 GLU CG C 13 31.33 0.05 . 1 . . . . . . . . 5894 1 318 . 1 1 84 84 SER H H 1 8.26 0.02 . 1 . . . . . . . . 5894 1 319 . 1 1 84 84 SER N N 15 115.63 0.05 . 1 . . . . . . . . 5894 1 320 . 1 1 84 84 SER CA C 13 59.49 0.05 . 1 . . . . . . . . 5894 1 321 . 1 1 84 84 SER HA H 1 4.42 0.02 . 1 . . . . . . . . 5894 1 322 . 1 1 84 84 SER C C 13 173.78 0.05 . 1 . . . . . . . . 5894 1 323 . 1 1 84 84 SER CB C 13 65.08 0.05 . 1 . . . . . . . . 5894 1 324 . 1 1 85 85 LYS H H 1 8.39 0.02 . 1 . . . . . . . . 5894 1 325 . 1 1 85 85 LYS N N 15 122.38 0.05 . 1 . . . . . . . . 5894 1 326 . 1 1 85 85 LYS CA C 13 57.47 0.05 . 1 . . . . . . . . 5894 1 327 . 1 1 85 85 LYS CB C 13 34.37 0.05 . 1 . . . . . . . . 5894 1 328 . 1 1 85 85 LYS CG C 13 25.74 0.05 . 1 . . . . . . . . 5894 1 329 . 1 1 86 86 ASP H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 330 . 1 1 86 86 ASP N N 15 119.76 0.05 . 1 . . . . . . . . 5894 1 331 . 1 1 86 86 ASP CA C 13 55.69 0.05 . 1 . . . . . . . . 5894 1 332 . 1 1 86 86 ASP HA H 1 4.54 0.02 . 1 . . . . . . . . 5894 1 333 . 1 1 86 86 ASP C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 334 . 1 1 86 86 ASP CB C 13 42.49 0.05 . 1 . . . . . . . . 5894 1 335 . 1 1 87 87 GLY H H 1 8.3 0.02 . 1 . . . . . . . . 5894 1 336 . 1 1 87 87 GLY N N 15 108.02 0.05 . 1 . . . . . . . . 5894 1 337 . 1 1 87 87 GLY CA C 13 46.82 0.05 . 1 . . . . . . . . 5894 1 338 . 1 1 87 87 GLY C C 13 173.34 0.05 . 1 . . . . . . . . 5894 1 339 . 1 1 87 87 GLY HA2 H 1 3.94 0.02 . 1 . . . . . . . . 5894 1 340 . 1 1 88 88 GLU H H 1 8.09 0.02 . 1 . . . . . . . . 5894 1 341 . 1 1 88 88 GLU N N 15 119.2 0.05 . 1 . . . . . . . . 5894 1 342 . 1 1 88 88 GLU CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 343 . 1 1 88 88 GLU C C 13 172.57 0.05 . 1 . . . . . . . . 5894 1 344 . 1 1 88 88 GLU CB C 13 37.67 0.05 . 1 . . . . . . . . 5894 1 345 . 1 1 88 88 GLU CG C 13 31.33 0.05 . 1 . . . . . . . . 5894 1 346 . 1 1 88 88 GLU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 347 . 1 1 89 89 GLY H H 1 8.5 0.02 . 1 . . . . . . . . 5894 1 348 . 1 1 89 89 GLY N N 15 108.87 0.05 . 1 . . . . . . . . 5894 1 349 . 1 1 89 89 GLY CA C 13 46.55 0.05 . 1 . . . . . . . . 5894 1 350 . 1 1 89 89 GLY HA2 H 1 3.94 0.02 . 1 . . . . . . . . 5894 1 351 . 1 1 90 90 ARG H H 1 8.19 0.02 . 1 . . . . . . . . 5894 1 352 . 1 1 90 90 ARG N N 15 117.45 0.05 . 1 . . . . . . . . 5894 1 353 . 1 1 90 90 ARG CA C 13 57.72 0.05 . 1 . . . . . . . . 5894 1 354 . 1 1 90 90 ARG C C 13 172.91 0.05 . 1 . . . . . . . . 5894 1 355 . 1 1 90 90 ARG CB C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 356 . 1 1 90 90 ARG CG C 13 31.83 0.05 . 1 . . . . . . . . 5894 1 357 . 1 1 91 91 GLU H H 1 8.24 0.02 . 1 . . . . . . . . 5894 1 358 . 1 1 91 91 GLU N N 15 120.96 0.05 . 1 . . . . . . . . 5894 1 359 . 1 1 91 91 GLU CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 360 . 1 1 91 91 GLU HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 361 . 1 1 91 91 GLU C C 13 172.79 0.05 . 1 . . . . . . . . 5894 1 362 . 1 1 91 91 GLU CB C 13 37.41 0.05 . 1 . . . . . . . . 5894 1 363 . 1 1 92 92 MET H H 1 8.29 0.02 . 1 . . . . . . . . 5894 1 364 . 1 1 92 92 MET N N 15 121.14 0.05 . 1 . . . . . . . . 5894 1 365 . 1 1 92 92 MET CA C 13 56.45 0.05 . 1 . . . . . . . . 5894 1 366 . 1 1 92 92 MET HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 367 . 1 1 92 92 MET C C 13 174 0.05 . 1 . . . . . . . . 5894 1 368 . 1 1 92 92 MET CB C 13 34.12 0.05 . 1 . . . . . . . . 5894 1 369 . 1 1 92 92 MET CG C 13 33.1 0.05 . 1 . . . . . . . . 5894 1 370 . 1 1 93 93 ALA H H 1 8.16 0.02 . 1 . . . . . . . . 5894 1 371 . 1 1 93 93 ALA N N 15 123.62 0.05 . 1 . . . . . . . . 5894 1 372 . 1 1 93 93 ALA CA C 13 53.91 0.05 . 1 . . . . . . . . 5894 1 373 . 1 1 93 93 ALA HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 374 . 1 1 93 93 ALA C C 13 175.42 0.05 . 1 . . . . . . . . 5894 1 375 . 1 1 93 93 ALA CB C 13 20.41 0.05 . 1 . . . . . . . . 5894 1 376 . 1 1 94 94 PHE H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 377 . 1 1 94 94 PHE N N 15 118.15 0.05 . 1 . . . . . . . . 5894 1 378 . 1 1 94 94 PHE CA C 13 56.71 0.05 . 1 . . . . . . . . 5894 1 379 . 1 1 94 94 PHE HA H 1 4.07 0.02 . 1 . . . . . . . . 5894 1 380 . 1 1 94 94 PHE C C 13 174.87 0.05 . 1 . . . . . . . . 5894 1 381 . 1 1 94 94 PHE CB C 13 42.49 0.05 . 1 . . . . . . . . 5894 1 382 . 1 1 95 95 GLU H H 1 8.35 0.02 . 1 . . . . . . . . 5894 1 383 . 1 1 95 95 GLU N N 15 120.08 0.05 . 1 . . . . . . . . 5894 1 384 . 1 1 95 95 GLU CA C 13 57.72 0.05 . 1 . . . . . . . . 5894 1 385 . 1 1 95 95 GLU HA H 1 4.16 0.02 . 1 . . . . . . . . 5894 1 386 . 1 1 95 95 GLU C C 13 174.87 0.05 . 1 . . . . . . . . 5894 1 387 . 1 1 95 95 GLU CB C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 388 . 1 1 95 95 GLU CG C 13 31.83 0.05 . 1 . . . . . . . . 5894 1 389 . 1 1 96 96 PHE H H 1 8.11 0.02 . 1 . . . . . . . . 5894 1 390 . 1 1 96 96 PHE N N 15 119.44 0.05 . 1 . . . . . . . . 5894 1 391 . 1 1 96 96 PHE CA C 13 58.74 0.05 . 1 . . . . . . . . 5894 1 392 . 1 1 96 96 PHE C C 13 171.81 0.05 . 1 . . . . . . . . 5894 1 393 . 1 1 96 96 PHE CB C 13 40.72 0.05 . 1 . . . . . . . . 5894 1 394 . 1 1 97 97 LEU H H 1 8.11 0.02 . 1 . . . . . . . . 5894 1 395 . 1 1 97 97 LEU N N 15 122.71 0.05 . 1 . . . . . . . . 5894 1 396 . 1 1 97 97 LEU CA C 13 56.19 0.05 . 1 . . . . . . . . 5894 1 397 . 1 1 97 97 LEU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 398 . 1 1 97 97 LEU C C 13 172.47 0.05 . 1 . . . . . . . . 5894 1 399 . 1 1 97 97 LEU CB C 13 43.76 0.05 . 1 . . . . . . . . 5894 1 400 . 1 1 97 97 LEU CG C 13 28.02 0.05 . 1 . . . . . . . . 5894 1 401 . 1 1 97 97 LEU CD1 C 13 26.25 0.05 . 1 . . . . . . . . 5894 1 402 . 1 1 97 97 LEU CD2 C 13 24.73 0.05 . 1 . . . . . . . . 5894 1 403 . 1 1 98 98 ASP H H 1 8.18 0.02 . 1 . . . . . . . . 5894 1 404 . 1 1 98 98 ASP N N 15 119.69 0.05 . 1 . . . . . . . . 5894 1 405 . 1 1 98 98 ASP CA C 13 55.69 0.05 . 1 . . . . . . . . 5894 1 406 . 1 1 98 98 ASP C C 13 173.67 0.05 . 1 . . . . . . . . 5894 1 407 . 1 1 98 98 ASP CB C 13 42.49 0.05 . 1 . . . . . . . . 5894 1 408 . 1 1 99 99 GLY H H 1 8.25 0.02 . 1 . . . . . . . . 5894 1 409 . 1 1 99 99 GLY N N 15 108.04 0.05 . 1 . . . . . . . . 5894 1 410 . 1 1 99 99 GLY CA C 13 46.81 0.05 . 1 . . . . . . . . 5894 1 411 . 1 1 99 99 GLY C C 13 173.23 0.05 . 1 . . . . . . . . 5894 1 412 . 1 1 99 99 GLY HA2 H 1 3.94 0.02 . 1 . . . . . . . . 5894 1 413 . 1 1 100 100 VAL H H 1 7.9 0.02 . 1 . . . . . . . . 5894 1 414 . 1 1 100 100 VAL N N 15 117.91 0.05 . 1 . . . . . . . . 5894 1 415 . 1 1 100 100 VAL CA C 13 64.06 0.05 . 1 . . . . . . . . 5894 1 416 . 1 1 100 100 VAL HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 417 . 1 1 100 100 VAL C C 13 174 0.05 . 1 . . . . . . . . 5894 1 418 . 1 1 100 100 VAL CB C 13 33.86 0.05 . 1 . . . . . . . . 5894 1 419 . 1 1 100 100 VAL CG1 C 13 22.18 0.05 . 1 . . . . . . . . 5894 1 420 . 1 1 101 101 ASN H H 1 8.45 0.02 . 1 . . . . . . . . 5894 1 421 . 1 1 101 101 ASN N N 15 120.22 0.05 . 1 . . . . . . . . 5894 1 422 . 1 1 101 101 ASN CA C 13 54.67 0.05 . 1 . . . . . . . . 5894 1 423 . 1 1 101 101 ASN HA H 1 4.67 0.02 . 1 . . . . . . . . 5894 1 424 . 1 1 101 101 ASN C C 13 172.68 0.05 . 1 . . . . . . . . 5894 1 425 . 1 1 101 101 ASN CB C 13 40.46 0.05 . 1 . . . . . . . . 5894 1 426 . 1 1 102 102 GLU H H 1 8.22 0.02 . 1 . . . . . . . . 5894 1 427 . 1 1 102 102 GLU N N 15 120.15 0.05 . 1 . . . . . . . . 5894 1 428 . 1 1 102 102 GLU CA C 13 57.97 0.05 . 1 . . . . . . . . 5894 1 429 . 1 1 102 102 GLU HA H 1 4.2 0.02 . 1 . . . . . . . . 5894 1 430 . 1 1 102 102 GLU C C 13 174 0.05 . 1 . . . . . . . . 5894 1 431 . 1 1 102 102 GLU CB C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 432 . 1 1 102 102 GLU CG C 13 31.58 0.05 . 1 . . . . . . . . 5894 1 433 . 1 1 103 103 VAL H H 1 8.12 0.02 . 1 . . . . . . . . 5894 1 434 . 1 1 103 103 VAL N N 15 120.79 0.05 . 1 . . . . . . . . 5894 1 435 . 1 1 103 103 VAL CA C 13 63.3 0.05 . 1 . . . . . . . . 5894 1 436 . 1 1 103 103 VAL HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 437 . 1 1 103 103 VAL C C 13 172.14 0.05 . 1 . . . . . . . . 5894 1 438 . 1 1 103 103 VAL CB C 13 34.11 0.05 . 1 . . . . . . . . 5894 1 439 . 1 1 103 103 VAL CG1 C 13 22.18 0.05 . 1 . . . . . . . . 5894 1 440 . 1 1 104 104 LYS H H 1 8.33 0.02 . 1 . . . . . . . . 5894 1 441 . 1 1 104 104 LYS N N 15 124.48 0.05 . 1 . . . . . . . . 5894 1 442 . 1 1 104 104 LYS CA C 13 56.95 0.05 . 1 . . . . . . . . 5894 1 443 . 1 1 104 104 LYS HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 444 . 1 1 104 104 LYS C C 13 173.78 0.05 . 1 . . . . . . . . 5894 1 445 . 1 1 104 104 LYS CB C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 446 . 1 1 104 104 LYS CG C 13 24.73 0.05 . 1 . . . . . . . . 5894 1 447 . 1 1 105 105 PHE H H 1 8.28 0.02 . 1 . . . . . . . . 5894 1 448 . 1 1 105 105 PHE N N 15 117.59 0.05 . 1 . . . . . . . . 5894 1 449 . 1 1 105 105 PHE CA C 13 54.67 0.05 . 1 . . . . . . . . 5894 1 450 . 1 1 105 105 PHE HA H 1 4.67 0.02 . 1 . . . . . . . . 5894 1 451 . 1 1 105 105 PHE C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 452 . 1 1 105 105 PHE CB C 13 39.95 0.05 . 1 . . . . . . . . 5894 1 453 . 1 1 106 106 GLU H H 1 8.21 0.02 . 1 . . . . . . . . 5894 1 454 . 1 1 106 106 GLU N N 15 121.18 0.05 . 1 . . . . . . . . 5894 1 455 . 1 1 106 106 GLU CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 456 . 1 1 106 106 GLU HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 457 . 1 1 106 106 GLU C C 13 172.9 0.05 . 1 . . . . . . . . 5894 1 458 . 1 1 106 106 GLU CB C 13 35.13 0.05 . 1 . . . . . . . . 5894 1 459 . 1 1 106 106 GLU CG C 13 30.56 0.05 . 1 . . . . . . . . 5894 1 460 . 1 1 107 107 ARG H H 1 8.32 0.02 . 1 . . . . . . . . 5894 1 461 . 1 1 107 107 ARG N N 15 120.75 0.05 . 1 . . . . . . . . 5894 1 462 . 1 1 107 107 ARG CA C 13 57.21 0.05 . 1 . . . . . . . . 5894 1 463 . 1 1 107 107 ARG HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 464 . 1 1 107 107 ARG C C 13 173.12 0.05 . 1 . . . . . . . . 5894 1 465 . 1 1 107 107 ARG CB C 13 32.08 0.05 . 1 . . . . . . . . 5894 1 466 . 1 1 107 107 ARG CG C 13 28.78 0.05 . 1 . . . . . . . . 5894 1 467 . 1 1 108 108 LEU H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 468 . 1 1 108 108 LEU N N 15 122.68 0.05 . 1 . . . . . . . . 5894 1 469 . 1 1 108 108 LEU CA C 13 56.19 0.05 . 1 . . . . . . . . 5894 1 470 . 1 1 108 108 LEU HA H 1 4.33 0.02 . 1 . . . . . . . . 5894 1 471 . 1 1 108 108 LEU C C 13 172.14 0.05 . 1 . . . . . . . . 5894 1 472 . 1 1 108 108 LEU CB C 13 43.76 0.05 . 1 . . . . . . . . 5894 1 473 . 1 1 108 108 LEU CG C 13 28.28 0.05 . 1 . . . . . . . . 5894 1 474 . 1 1 108 108 LEU CD1 C 13 26.25 0.05 . 1 . . . . . . . . 5894 1 475 . 1 1 108 108 LEU CD2 C 13 24.72 0.05 . 1 . . . . . . . . 5894 1 476 . 1 1 109 109 VAL H H 1 8.06 0.02 . 1 . . . . . . . . 5894 1 477 . 1 1 109 109 VAL N N 15 121.3 0.05 . 1 . . . . . . . . 5894 1 478 . 1 1 109 109 VAL CA C 13 63.81 0.05 . 1 . . . . . . . . 5894 1 479 . 1 1 109 109 VAL HA H 1 4.37 0.02 . 1 . . . . . . . . 5894 1 480 . 1 1 109 109 VAL C C 13 173.89 0.05 . 1 . . . . . . . . 5894 1 481 . 1 1 109 109 VAL CB C 13 33.61 0.05 . 1 . . . . . . . . 5894 1 482 . 1 1 109 109 VAL CG1 C 13 22.44 0.05 . 1 . . . . . . . . 5894 1 483 . 1 1 110 110 LYS H H 1 8.23 0.02 . 1 . . . . . . . . 5894 1 484 . 1 1 110 110 LYS N N 15 123.81 0.05 . 1 . . . . . . . . 5894 1 485 . 1 1 110 110 LYS CA C 13 57.72 0.05 . 1 . . . . . . . . 5894 1 486 . 1 1 110 110 LYS HA H 1 4.28 0.02 . 1 . . . . . . . . 5894 1 487 . 1 1 110 110 LYS C C 13 174.98 0.05 . 1 . . . . . . . . 5894 1 488 . 1 1 110 110 LYS CB C 13 31.57 0.05 . 1 . . . . . . . . 5894 1 489 . 1 1 111 111 GLU H H 1 8.17 0.02 . 1 . . . . . . . . 5894 1 490 . 1 1 111 111 GLU N N 15 119.33 0.05 . 1 . . . . . . . . 5894 1 491 . 1 1 111 111 GLU CA C 13 58.99 0.05 . 1 . . . . . . . . 5894 1 492 . 1 1 111 111 GLU HA H 1 4.59 0.02 . 1 . . . . . . . . 5894 1 493 . 1 1 111 111 GLU C C 13 173.56 0.05 . 1 . . . . . . . . 5894 1 494 . 1 1 111 111 GLU CG C 13 40.97 0.05 . 1 . . . . . . . . 5894 1 495 . 1 1 112 112 GLU H H 1 8.33 0.02 . 1 . . . . . . . . 5894 1 496 . 1 1 112 112 GLU N N 15 121.48 0.05 . 1 . . . . . . . . 5894 1 497 . 1 1 112 112 GLU CA C 13 57.72 0.05 . 1 . . . . . . . . 5894 1 498 . 1 1 112 112 GLU HA H 1 4.24 0.02 . 1 . . . . . . . . 5894 1 499 . 1 1 112 112 GLU C C 13 173.23 0.05 . 1 . . . . . . . . 5894 1 500 . 1 1 112 112 GLU CB C 13 37.42 0.05 . 1 . . . . . . . . 5894 1 501 . 1 1 112 112 GLU CG C 13 31.57 0.05 . 1 . . . . . . . . 5894 1 502 . 1 1 113 113 LYS H H 1 7.94 0.02 . 1 . . . . . . . . 5894 1 503 . 1 1 113 113 LYS N N 15 126.5 0.05 . 1 . . . . . . . . 5894 1 504 . 1 1 113 113 LYS CA C 13 58.88 0.05 . 1 . . . . . . . . 5894 1 505 . 1 1 113 113 LYS HA H 1 4.16 0.02 . 1 . . . . . . . . 5894 1 506 . 1 1 113 113 LYS C C 13 174.32 0.05 . 1 . . . . . . . . 5894 1 507 . 1 1 113 113 LYS CB C 13 35.12 0.05 . 1 . . . . . . . . 5894 1 stop_ save_