BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4189

Title: Solution Structure of Reduced Horse Heart Cytochrome c

Authors: Banci, L.; Bertini, I.; Huber, J.; Spyroulias, G.; Turano, P.

Citation: Banci, L.; Bertini, I.; Huber, J.; Spyroulias, G.; Turano, P.. "Solution Structure of Reduced Horse Heart Cytochrome C"  J. Biol. Inorg. Chem. 4, 21-31 (1999).

Assembly members:
cytochrome c, polymer, 104 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:   Production method: .

Entity Sequences (FASTA):
cytochrome c: GDVEKGKKIFVQKCAQCHTV EKGGKHKTGPNLHGLFGRKT GQAPGFTYTDANKNKGITWK EETLMEYLENPKKYIPGTKM IFAGIKKKTEREDLIAYLKK ATNE

Data typeCount
1H chemical shifts709

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hh cytc1
2heme2

Entities:

Entity 1, hh cytc 104 residues - Formula weight is not available

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: cytochrome c2.5 – 3.0 mM; phosphate 100 mM

sample_two: cytochrome c2.5 – 3.0 mM; phosphate 100 mM

sample_conditions_one: pH: 7.0; pressure: 1013 mbar; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions_one
TOCSYsample_onenot availablesample_conditions_one

Software:

xwinnmr - Multiplication of raw data by a pure cosine-squared function Fourier Transform Polynomial baseline correction

XEASY - Volume integration of cross-peaks on 2D spectra

NMR spectrometers:

  • BRUKER AVANCE 800 MHz
  • BRUKER AVANCE 600 MHz

Related Database Links:

BMRB 1058 1107 1108 1109 1110 1111 1112 1113 1114 1116 1170 1171 1404 16759 17120 17340 1736 1783 17847 17848 1785 1787 1789 216 220 224 2366 2367 2368 243 244 25640 274 285 286 316 317 336 436 437 438 439
PDB
GB AAB33495 AAI05398 AAX77008 ABA06541 AEB61027
PRF 610169A 711086A
REF NP_001039526 NP_001123442 NP_001157486 XP_004007999 XP_004418964
SP P00004 P62894 P62895 P62896 P68096
TPG DAA30512